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CETP Antibody (MA1-050) in ICC/IF
Immunofluorescent analysis of CETP in HepG2 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a CETP monoclonal antibody (Product # MA1-050) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). CETP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
产品信息
MA1-050
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Rabbit
已发表种属
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
ATM192
抗原
Synthetic peptide corresponding to residues C D(149) S G R V R T D A P D(159) of human CETP protein.
偶联物
形式
Liquid
浓度
2 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_325404
产品详细信息
MA1-050 detects cholesteryl ester transfer protein (CETP) from human and rabbit tissues.
MA1-050 has been successfully used in Western blot, immunoprecipitation, IF and ELISA protocols. By Western blot, this antibody detects a doublet at ~70 kDa from human serum representing CETP. This antibody can also be used to immunoprecipitate CETP activity from serum samples using protein G.
The MA1-050 immunogen is a synthetic peptide corresponding to residues C D(149) S G R V R T D A P D(159) of human CETP protein.
靶标信息
It has been proposed that high density lipoproteins (HDLs) function jointly with lecithin:cholesterol acyltransferase and CETP to facilitate cholesterol transport from tissues to the liver. This mechanism, referred to as reverse cholesterol transport, is physiologically important because it maintains systemic cholesterol levels. CETP is responsible for neutral lipid transfer activity in plasma in numerous species. Since CETP is able to accelerate specifically the exchange of lipid components between pro- and anti-atherogenic lipoprotein fractions, it may be a key determinant of the global atherogenicity of the plasma lipoprotein profile and arises as a possible target in atherosclerosis prevention. CETP has an important role in reverse cholesterol transport and shaping and affecting the composition of plasma lipoproteins. In general elevated levels of CETP have been associated with increased risk of coronary heart disease.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: BPI fold containing family F; Cholesteryl ester transfer protein; cholesteryl ester transfer protein plasma; cholesteryl ester transfer protein, plasma; Lipid transfer protein I
基因别名: BPIFF; CETP; HDLCQ10
UniProt ID: (Human) P11597
Entrez Gene ID: (Human) 1071, (Rabbit) 100327267
triglyceride metabolic process
lipid transport
receptor-mediated endocytosis
cholesterol metabolic process
negative regulation of macrophage derived foam cell differentiation
regulation of cholesterol efflux
phospholipid transport
cholesterol transport
triglyceride transport
very-low-density lipoprotein particle remodeling
low-density lipoprotein particle remodeling
high-density lipoprotein particle remodeling
lipoprotein metabolic process
cholesterol homeostasis
reverse cholesterol transport
phosphatidylcholine metabolic process
lipid homeostasis
phospholipid homeostasis
triglyceride homeostasis
