Invitrogen

CHOP Polyclonal Antibody

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抗体检测数据 (2)
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CHOP Antibody in Western Blot (WB)

CHOP Antibody in Western Blot (WB)

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CHOP Antibody (PA5-35129) in WB

Western blot analysis of CHOP in Jurkat cell line lysates. Samples were incubated with CHOP polyclonal antibody (Product # PA5-35129). Lysates: 35 µg/lane. DDIT3 protein (arrow).

CHOP Antibody in Western Blot (WB)

CHOP Antibody in Flow Cytometry (Flow)

CHOP Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CHOP Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

CHOP Antibody in Western Blot (WB) — Western blot analysis of CHOP in Jurkat cell line lysates. Samples were incubated with CHOP polyclonal antibody (Product # PA5-35129). Lysates: 35 µg/lane. DDIT3 protein (arrow).

CHOP Antibody in Flow Cytometry (Flow) — Flow cytometry of CHOP in Jurkat cells (right histogram). Samples were incubated with CHOP polyclonal antibody (Product # PA5-35129) followed by FITC-conjugated donkey-anti-rabbit secondary antibody. Negative control cell (left histogram).

CHOP Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow) — Figure 5 (A) Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of DEGs (p < 0.05). (B) Representative TEM picture in survival and death. (C) Representative photomicrographs showing SDF4 (green), DAPI (blue) and their merged images (original magnification x400). Scale bar, 100um. (D) GRP78 expression in survival (n=68) compared with death (n=21) in cohort. (E) Pearson correlation analysis between the expression level of GRP78 and SDF4 in cohort. (F) Pearson correlation analysis between the expression level of ATF6 and SDF4 in cohort. (G) Representative histograms of CHOP expression among survival and death in flow cytometry. (H) Mean fluorescence intensity of CHOP in survival (n=54) and death (n=19). (I) Representative histograms of ATF6 expression among survival and death in flow cytometry. (J) Mean fluorescence intensity of ATF6 in survival and death. (K) Representative histograms of GRP78 expression among survival and death in flow cytometry. (L) Mean fluorescence intensity of GRP78 in survival and death. (M) Representative photomicrographs showing CHOP (red), DAPI (blue) and their merged images (original magnification x400). Scale bar, 100um. (N) Representative photomicrographs showing ATF6 (green), DAPI (blue) and their merged images (original magnification x400). (O) Representative photomicrographs showing GRP78 (red), DAPI (blue) and their merged images (original magnification x400). ***p < 0.001, **p < 0.01, *p < 0.05.

CHOP Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Figure 7 (A) Representative photomicrographs of Grp78 and Chop from sham-operated and CLP mice lung treated with AdCon or AdSdf4 (original magnification x400). Scale bar, 100um. (B, C) Positive staining area of Grp78 and Chop from four groups. (D) Representative western blot results from four groups for levels of cleaved- Atf6 , Grp78 , Chop , and beta-actin. (E-G) The cleaved- Atf6 , Grp78 and Chop bands were quantified by densitometry and normalized to the density of beta-actin. n=5. Data were shown in Mean +- SD. ****p < 0.001, ***p < 0.001, **p < 0.01, *p < 0.05.

产品信息

PA5-35129

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化 (IHC)

-

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免疫细胞化学 (ICC/IF)

-

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流式细胞分析 (Flow)

1:10-1:50

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 82-110 amino acids from the central region of human DDIT3

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2552439

靶标信息

GADD153 is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP GADD153 dimer gains binding activity to other non classical C/EBP stress related targets. Under normal cellular conditions this protein is not expressed in detectable levels, but is highly unregulated during times of cellular/ER stress. Examples of GADD153 inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

SDF4 Is a Prognostic Factor for 28-Days Mortality in Patients With Sepsis via Negatively Regulating ER Stress.

Frontiers in immunology

Zhu T,Su Q,Wang C,Shen L,Chen H,Feng S,Peng X,Chen S,Wang Y,Jiang H,Chen J

PA5-35129 was used in Flow Cytometry to indicate that incorporation of SDF4 can improve clinical parameters predictive value for the prognosis of sepsis, and decreased expression levels of SDF4 contributes to excessive ER stress, which is associated with worsened outcomes, whereas overexpression of SDF4 attenuated such activation.

Thu Sep 30 00:00:00 EDT 2021

SDF4 Is a Prognostic Factor for 28-Days Mortality in Patients With Sepsis via Negatively Regulating ER Stress.

Frontiers in immunology

Zhu T,Su Q,Wang C,Shen L,Chen H,Feng S,Peng X,Chen S,Wang Y,Jiang H,Chen J

PA5-35129 was used in Flow Cytometry to indicate that incorporation of SDF4 can improve clinical parameters predictive value for the prognosis of sepsis, and decreased expression levels of SDF4 contributes to excessive ER stress, which is associated with worsened outcomes, whereas overexpression of SDF4 attenuated such activation.

Thu Sep 30 00:00:00 EDT 2021

SDF4 Is a Prognostic Factor for 28-Days Mortality in Patients With Sepsis via Negatively Regulating ER Stress.

Frontiers in immunology

Zhu T,Su Q,Wang C,Shen L,Chen H,Feng S,Peng X,Chen S,Wang Y,Jiang H,Chen J

PA5-35129 was used in Flow Cytometry to indicate that incorporation of SDF4 can improve clinical parameters predictive value for the prognosis of sepsis, and decreased expression levels of SDF4 contributes to excessive ER stress, which is associated with worsened outcomes, whereas overexpression of SDF4 attenuated such activation.

Thu Sep 30 00:00:00 EDT 2021

SDF4 Is a Prognostic Factor for 28-Days Mortality in Patients With Sepsis via Negatively Regulating ER Stress.

Frontiers in immunology

Zhu T,Su Q,Wang C,Shen L,Chen H,Feng S,Peng X,Chen S,Wang Y,Jiang H,Chen J

PA5-35129 was used in Flow Cytometry to indicate that incorporation of SDF4 can improve clinical parameters predictive value for the prognosis of sepsis, and decreased expression levels of SDF4 contributes to excessive ER stress, which is associated with worsened outcomes, whereas overexpression of SDF4 attenuated such activation.

Thu Sep 30 00:00:00 EDT 2021

SDF4 Is a Prognostic Factor for 28-Days Mortality in Patients With Sepsis via Negatively Regulating ER Stress.

Frontiers in immunology

Zhu T,Su Q,Wang C,Shen L,Chen H,Feng S,Peng X,Chen S,Wang Y,Jiang H,Chen J

PA5-35129 was used in Flow Cytometry to indicate that incorporation of SDF4 can improve clinical parameters predictive value for the prognosis of sepsis, and decreased expression levels of SDF4 contributes to excessive ER stress, which is associated with worsened outcomes, whereas overexpression of SDF4 attenuated such activation.

Thu Sep 30 00:00:00 EDT 2021

生物信息学

蛋白别名: C/EBP zeta; C/EBP-homologous protein; C/EBP-homologous protein 10; CCAAT/enhancer-binding protein homologous protein; CHOP; CHOP-10; DDIT-3; DNA damage-inducible transcript 3 protein; DNA-damage-inducible transcript 3; Growth arrest and DNA damage-inducible protein GADD153; MGC4154

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基因别名: CEBPZ; CHOP; CHOP-10; CHOP10; DDIT3; GADD153

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UniProt ID: (Human) P35638

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Entrez Gene ID: (Human) 1649

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transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein binding transcription factor binding cAMP response element binding protein binding protein homodimerization activity leucine zipper domain binding transcription regulatory region DNA binding protein heterodimerization activity

参与通路

negative regulation of transcription from RNA polymerase II promoter blood vessel maturation response to amphetamine regulation of transcription, DNA-templated cellular response to DNA damage stimulus ER overload response response to unfolded protein cell cycle arrest aging response to nutrient Wnt signaling pathway positive regulation of interleukin-8 production negative regulation of CREB transcription factor activity response to endoplasmic reticulum stress PERK-mediated unfolded protein response ATF6-mediated unfolded protein response response to drug response to hydrogen peroxide response to starvation mRNA transcription from RNA polymerase II promoter proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of sequence-specific DNA binding transcription factor activity positive regulation of neuron apoptotic process regulation of DNA-templated transcription in response to stress regulation of transcription involved in anterior/posterior axis specification cell redox homeostasis negative regulation of fat cell differentiation negative regulation of myoblast differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic organ development release of sequestered calcium ion into cytosol negative regulation of protein kinase B signaling intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress negative regulation of canonical Wnt signaling pathway positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress negative regulation of determination of dorsal identity