Proteintech

CHOP/GADD153 Recombinant Rabbit Monoclonal Antibody (1G11)

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CHOP/GADD153 Antibody in Western Blot (WB)

CHOP/GADD153 Antibody in Western Blot (WB)

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CHOP/GADD153 Antibody (81462-1-RR) in WB

Untreated and tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 81462-1-RR (CHOP; GADD153 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

CHOP/GADD153 Antibody in Western Blot (WB)

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CHOP/GADD153 Antibody in Western Blot (WB) — Untreated and tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 81462-1-RR (CHOP; GADD153 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

产品信息

81462-1-RR

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000-1:10,000

-

酶联免疫吸附实验 (ELISA)

Assay-dependent

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Mammalian cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

1G11

抗原

Recombinant protein

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Protein A

保存液

PBS with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

产品详细信息

Immunogen sequence: MAAESLPFSF GTLSSWELEA WYEDLQEVLS SDENGGTYVS PPGNEEEESK IFTTLDPASL AWLTEEEPEP AEVTSTSQSP HSPDSSQSSL AQEEEEEDQG RTRKRKQSGH SPARAGKQRM KEKEQENERK VAQLAEENER LKQEIERLTR EVEATRRALI DRMVNLHQA

靶标信息

GADD153 is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP GADD153 dimer gains binding activity to other non classical C/EBP stress related targets. Under normal cellular conditions this protein is not expressed in detectable levels, but is highly unregulated during times of cellular/ER stress. Examples of GADD153 inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: C/EBP zeta; C/EBP-homologous protein; C/EBP-homologous protein 10; CCAAT/enhancer-binding protein homologous protein; CHOP; CHOP-10; DDIT-3; DNA damage-inducible transcript 3 protein; DNA-damage-inducible transcript 3; Growth arrest and DNA damage-inducible protein GADD153; MGC4154

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基因别名: CEBPZ; CHOP; CHOP-10; CHOP10; DDIT3; GADD153

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UniProt ID: (Human) P35638

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Entrez Gene ID: (Human) 1649

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transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein binding transcription factor binding cAMP response element binding protein binding protein homodimerization activity leucine zipper domain binding transcription regulatory region DNA binding protein heterodimerization activity

参与通路

negative regulation of transcription from RNA polymerase II promoter blood vessel maturation response to amphetamine regulation of transcription, DNA-templated cellular response to DNA damage stimulus ER overload response response to unfolded protein cell cycle arrest aging response to nutrient Wnt signaling pathway positive regulation of interleukin-8 production negative regulation of CREB transcription factor activity response to endoplasmic reticulum stress PERK-mediated unfolded protein response ATF6-mediated unfolded protein response response to drug response to hydrogen peroxide response to starvation mRNA transcription from RNA polymerase II promoter proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of sequence-specific DNA binding transcription factor activity positive regulation of neuron apoptotic process regulation of DNA-templated transcription in response to stress regulation of transcription involved in anterior/posterior axis specification cell redox homeostasis negative regulation of fat cell differentiation negative regulation of myoblast differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic organ development release of sequestered calcium ion into cytosol negative regulation of protein kinase B signaling intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress negative regulation of canonical Wnt signaling pathway positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress negative regulation of determination of dorsal identity