Invitrogen

CK2 alpha-2 Monoclonal Antibody (1E1)

CK2 alpha-2 Antibody in Western Blot (WB)

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CK2 alpha-2 Antibody (MA5-17062) in WB

Western blot analysis of CSNK2A2 using CSNK2A2 monoclonal antibody (Product # MA5-17062) in HeLa (1), MCF-7 (2), HepG2 (3), Jurkat (4), NIH3T3 (5), and PC-12 (6) cell lysate.

CK2 alpha-2 Antibody in Flow Cytometry (Flow)

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产品信息

MA5-17062

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

1:200-1:400

酶联免疫吸附实验 (ELISA)

1:10,000

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

1E1

抗原

Purified recombinant fragment of human CSNK2A2 (amino acids: 194-350) expressed in E. Coli.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

ascites

内含物

0.03% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2538533

产品详细信息

MA5-17062 targets CSNK2A2 in FACS, indirect ELISA, and WB applications and shows reactivity with Human and Mouse samples.

The MA5-17062 immunogen is purified recombinant fragment of human CSNK2A2 (amino acids: 194-350) expressed in E. Coli.

MA5-17062 detects CSNK2A2 which has a predicted molecular weight of approximately 41.2kDa.

靶标信息

Casein kinase II is a protein serine/threonine kinase that is thought to be present in all eukarotic cells, implying that it has fundamental cellular functions. The holoenzyme is a tetramer containing 2 alpha or alpha-prime subunits (or one of each) and 2 beta subunits. The function of beta subunits is unknown and the alpha subunit is the catalytic unit. CK2 functions as a tetrameric complex consisting of two regulatory beta-subunits and two catalytic units (alpha and alpha') in a homomeric or heteromeric conformation. Whilst the alpha- and alpha'-subunits are catalytically identical, proteins that regulate CK2, such as cdc2 and Hsp90, preferentially bind to the alpha and not the alpha'-subunit. CK2 can phosphorylate a number of key intracellular signaling proteins implicated in tumor suppression (p53 and PTEN) and tumorigenesis (myc, jun, NF-kappaB). CK2 is also thought to influence Wnt signaling via beta-catenin phosphorylation and the PI 3-K signaling pathway via the phosphorylation of Akt.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Indomethacin Induces Spermidine/Spermine-N¹-Acetyltransferase-1 via the Nucleolin-CDK1 Axis and Synergizes with the Polyamine Oxidase Inhibitor Methoctramine in Lung Cancer Cells.

Biomolecules

Buelvas N,Ugarte-Vio I,Asencio-Leal L,Muñoz-Uribe M,Martin-Martin A,Rojas-Fernández A,Jara JA,Tapia JC,Arias ME,López-Muñoz RA

MA5-17062 was used in Western Blot to investigate the influence of the PPAR-γ on the SSAT-1 expression in two lung cancer cell lines: H1299 and A549.

Tue Sep 12 00:00:00 EDT 2023

Identification of potential candidate proteins for reprogramming spinal cord-derived astrocytes into neurons: a proteomic analysis.

Neural regeneration research

Chen WH,Lin YX,Lin L,Zhang BQ,Xu SX,Wang W

MA5-17062 was used in Western Blotting to reprogram reactive astrocytes from newborn rat spinal cords into neurons in vitro through the overexpression of NEUROD1.

Mon Nov 01 00:00:00 EDT 2021

Identification of potential candidate proteins for reprogramming spinal cord-derived astrocytes into neurons: a proteomic analysis.

Neural regeneration research

Chen WH,Lin YX,Lin L,Zhang BQ,Xu SX,Wang W

MA5-17062 was used in Western Blotting to reprogram reactive astrocytes from newborn rat spinal cords into neurons in vitro through the overexpression of NEUROD1.

Mon Nov 01 00:00:00 EDT 2021

生物信息学

蛋白别名: casein kinase 2 alpha; casein kinase 2 alpha'; casein kinase 2, alpha prime polypeptide; Casein kinase II subunit alpha'; casein kinase II, alpha 2, polypeptide; CK II alpha'

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基因别名: 1110035J23Rik; C77789; CK2; CK2A2; CK2alpha'; CSNK2A1; CSNK2A2

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UniProt ID: (Human) P19784, (Mouse) O54833

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Entrez Gene ID: (Human) 1459, (Mouse) 13000, (Rat) 307641

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功能

protein serine/threonine kinase activity protein binding ATP binding protein N-terminus binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

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