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CNOT8 Antibody (PA5-100445) in ICC/IF
Immunocytochemistry analysis of CNOT8 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with CNOT8 polyclonal antibody (Product # PA5-100445) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-100445
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human CNOT8(Accession Q9UFF9), corresponding to amino acid residues P89-L139.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2849955
产品详细信息
Antibody detects endogenous levels of total CNOT8.
靶标信息
CNOT8 (CCR4-NOT transcription complex subunit 8), also known as CALIF or POP2, is a 292 amino acid protein that localizes to both the nucleus and the cytoplasm and functions as part of the CCR-NOT complex. Expressed ubiquitously, CNOT8 plays a role in transcriptional regulation for a diverse set of processes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CAF1-like protein; Caf1b; CAF2; CALIFp; CCR4-associated factor 8; CCR4-NOT transcription complex subunit 8; CCR4-NOT transcription complex, subunit 8; h CAF1-like protein; PGK promoter directed over production
基因别名: 1500015I04Rik; 1810022F04Rik; AA536816; AU015770; AU043059; CAF1; Caf1b; CALIF; CNOT8; hCAF1; POP2
UniProt ID: (Human) B2RAR8, (Mouse) Q9D8X5
Entrez Gene ID: (Human) 9337, (Mouse) 69125, (Rat) 363603
3'-5'-exoribonuclease activity
transcription factor activity, sequence-specific DNA binding
RNA binding
poly(A)-specific ribonuclease activity
protein binding
metal ion binding
nucleic acid binding
nuclease activity
exonuclease activity
hydrolase activity
molecular_function
mRNA polyadenylation factor
nuclear-transcribed mRNA poly(A) tail shortening
transcription, DNA-templated
regulation of transcription, DNA-templated
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
positive regulation of cell proliferation
negative regulation of cell proliferation
gene silencing by miRNA
exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay
positive regulation of mRNA catabolic process
RNA phosphodiester bond hydrolysis, exonucleolytic
regulation of translation
gene silencing by RNA
nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
biological_process
