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CNOT8 Antibody (PA5-13451) in ICC/IF
Immunocytochemistry analysis of CNOT8 in HeLa cells. Samples were incubated with CNOT8 polyclonal antibody (Product # PA5-13451) using a dilution of 1:25 for 1 h at 37°C followed by Alexa Fluor® 488 conjugated donkey anti-rabbit at a dilution of 1:400 for 50 min at 37°C. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min). CNOT8... View More
产品信息
PA5-13451
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
KLH conjugated synthetic peptide between 227-255 amino acids from the C-terminal region of human CNOT8
偶联物
形式
Liquid
浓度
0.4 mg/mL
纯化类型
Protein A, Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2082471
产品详细信息
This antibody is predicted to react with mouse based on sequence homology.
靶标信息
CNOT8 (CCR4-NOT transcription complex subunit 8), also known as CALIF or POP2, is a 292 amino acid protein that localizes to both the nucleus and the cytoplasm and functions as part of the CCR-NOT complex. Expressed ubiquitously, CNOT8 plays a role in transcriptional regulation for a diverse set of processes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CAF1-like protein; Caf1b; CAF2; CALIFp; CCR4-associated factor 8; CCR4-NOT transcription complex subunit 8; CCR4-NOT transcription complex, subunit 8; h CAF1-like protein; PGK promoter directed over production
基因别名: CAF1; Caf1b; CALIF; CNOT8; hCAF1; POP2
UniProt ID: (Human) Q9UFF9
Entrez Gene ID: (Human) 9337
nuclear-transcribed mRNA poly(A) tail shortening
transcription, DNA-templated
regulation of transcription, DNA-templated
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
positive regulation of cell proliferation
negative regulation of cell proliferation
gene silencing by miRNA
exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay
positive regulation of mRNA catabolic process
RNA phosphodiester bond hydrolysis, exonucleolytic
