Invitrogen

COL6A6 Polyclonal Antibody

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4 篇参考文献

抗体检测数据 (4)
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COL6A6 Antibody in Immunohistochemistry (IHC)

COL6A6 Antibody in Immunohistochemistry (IHC)

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COL6A6 Antibody (PA5-60958) in IHC

Immunohistochemical staining of COL6A6 in human lung using COL6A6 Polyclonal Antibody (Product # PA5-60958) shows moderate to strong membranous positivity in pneumocytes.

COL6A6 Antibody in Immunohistochemistry (IHC)

COL6A6 Antibody in Immunohistochemistry (IHC)

COL6A6 Antibody in Immunohistochemistry (IHC)

COL6A6 Antibody in Immunohistochemistry (IHC)

COL6A6 Antibody in Immunohistochemistry (IHC)

COL6A6 Antibody in Immunohistochemistry (IHC)

COL6A6 Antibody in Western Blot (WB)

COL6A6 Antibody in Western Blot (WB)

COL6A6 Antibody in Western Blot (WB)

COL6A6 Antibody in Western Blot (WB)

COL6A6 Antibody in Western Blot (WB)

COL6A6 Antibody in Western Blot (WB)

Product Image Gallery

COL6A6 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of COL6A6 in human lung using COL6A6 Polyclonal Antibody (Product # PA5-60958) shows moderate to strong membranous positivity in pneumocytes.

COL6A6 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of COL6A6 in human pancreas using COL6A6 Polyclonal Antibody (Product # PA5-60958) shows very weak positivity in exocrine glandular cells.

COL6A6 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of COL6A6 in human breast using COL6A6 Polyclonal Antibody (Product # PA5-60958) shows moderate positivity in extracellular matrix.

COL6A6 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of COL6A6 in human skin using COL6A6 Polyclonal Antibody (Product # PA5-60958) shows moderate positivity in extracellular matrix.

COL6A6 Antibody in Immunohistochemistry (IHC) — The qPCR ( A ) and IHC ( B ) results showed that FOXJ1, MXRA5 and CXCL9 expression was low and that KIF26B, VSIG4 and COL6A6 expression was high in OC tissues

COL6A6 Antibody in Immunohistochemistry (IHC) — Figure 5 Collagen protein expression in HNSCC patients' tissues. COL3A1 and COL6A6 protein expression was evaluated in CAF-P ( A ) and CAF-D ( B ) tissues from patients with HNSCC.

COL6A6 Antibody in Western Blot (WB) — Figure 1 The expression of COL6A6 in NSCLC tissues. (A) COL6A6 is differentially expressed in tumors compared to normal tissues according to the GEPIA database. (B) The expression of COL6A6 in LUAD and LUSC in TCGA database (P<0.001). (C) Western blotting analysis of COL6A6 in NSCLC and adjacent tissues was performed. (D) The expression of COL6A6 in the immortalized bronchial epithelial cell line (BEAS-2B) and 4 NSCLC cell lines (H520, A549, H1299, and H1975) was analyzed by Western blotting. (E) The Kaplan-Meier plotter was used to analyze the overall survival, which showed that the prognosis of patients with high COL6A6 expression (red) was better than that of patients with low COL6A6 expression (blue). Data are expressed as mean +- SE. *, P<0.05; ***, P<0.001. N, normal tissue; T, tumor tissue; COL6A6, collagen type VI alpha 6 chain; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; TCGA, The Cancer Genome Atlas; GEPIA, Gene Expression Profiling Interactive Analysis; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; NSCLC, non-small cell lung cancer; SE, standard error.

COL6A6 Antibody in Western Blot (WB) — Figure 2 Verification of the transfection efficiency of COL6A6 knockdown in NSCLC cells. (A) Lentiviral siRNA targeting COL6A6 was transfected into H1299 cells. (B) More than 80% of the cells were green fluorescent protein (GFP) positive and were transfected successfully (x100). Scale bar: 100 um. (C) Western blotting assays were used to detect the expression of COL6A6 in H1299 cells transfected with siRNA. NC: uninfected H1299 cells; NC-si: negative control siRNA transfected H1299 cells; COL6A6-si: COL6A6 siRNA transfected H1299 cells. Data are expressed as mean +- SE. **, P<0.01; ***, P<0.001. COL6A6, collagen type VI alpha 6 chain; NSCLC, non-small cell lung cancer; SE, standard error.

COL6A6 Antibody in Western Blot (WB) — Figure 3 The effect of COL6A6 on the proliferation, invasion, migration, and EMT of AtT-20 and HP75 cells . ( A ) The expression of COL6A6 protein was determined by western blotting; ( B ) The proliferation ability of AtT-20 and HP75 cells were measured by CCK-8 assay; ( C and D ) The invasion of AtT-20 and HP75 cells were measured by Transwell assay (40x); ( E - G ) The migration ability of AtT-20 and HP75 cells were evaluated by wound healing assay (4x); ( H - K ) The expression of EMT related proteins in AtT-20 and HP75 cells were detected by western blotting. * P<0.05, ** P<0.01, *** P<0.001, compared with the NC group.

COL6A6 Antibody in Western Blot (WB) — Figure 2 The expression of COL6A6 and P4HA3 in PA tissues and cell lines. ( A and B ) RT-qPCR was used to detect the expression of COL6A6 and P4HA3 in PA tissues and normal pituitary tissues; ( C and D ) Immunohistochemistry staining was applied to detect the expression of COL6A6 and P4HA3 in PA tissues and normal pituitary tissues; ( E and F ) Western blotting was used to evaluate the expression of COL6A6 and P4HA3 in PA cell lines. *** P<0.001, compared with normal pituitary tissues; ## P<0.01, compared with AtT-20 cells; ^^ P<0.01, compared with HP75 cells.

COL6A6 Antibody in Western Blot (WB) — Figure 4 The effect of COL6A6 on the growth and metastasis of xenograft tumor . ( A and B ) The tumor size was obtained from nude mice; ( C and D ) Tumor volume and tumor weight of nude mice were analyzed; ( E and F ) The expression of COL6A6 in xenograft tumor tissues were detected by western blotting; ( G and H ) The expression of Ki-67 in xenograft tumor tissues were detected by immunohistochemistry staining; ( I and J ) The expression of E-cadherin, vimentin, and N-cadherin were measured by western blotting. ** P<0.01, *** P<0.001, compared with NC group.

COL6A6 Antibody in Western Blot (WB) — Figure 5 Effect of COL6A6 on the PI3K-Akt signaling pathway through interacting with P4HA3 . ( A and B ) Western blotting was used to detect the expression of COL6A6, P4HA3, PI3K-Akt pathway-related protein in AtT-20 and HP75 cells; ( C and D ) Coimmunoprecipitation to validate the COL6A6-P4HA3 interaction. ** P<0.01, *** P<0.001, compared with NC group; ## P<0.01, compared with COL6A6 group.

产品信息

PA5-60958

应用

建议稀释比

已发表文章

免疫印迹 (WB)

-

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免疫组化 (IHC)

1:50-1:200

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human COL6A6. Recombinant protein control fragment (Product #RP-100812).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2640027

产品详细信息

Immunogen sequence: ALREVEHYFR PDMGSRINTG TPQVLLVLTD GQSQDEVAQA AEALRHRGID IYSVGIGDVD DQQLIQITGT AEKKLTVHNF DELKKVNKRI VRN

Highest antigen sequence identity to the following orthologs: Mouse - 87%, Rat - 90%.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Establishment and validation of a novel invasion-related gene signature for predicting the prognosis of ovarian cancer.

Cancer cell international

Liang L,Li J,Yu J,Liu J,Xiu L,Zeng J,Wang T,Li N,Wu L

Published figure using COL6A6 polyclonal antibody (Product # PA5-60958) in Immunohistochemistry

Tue Mar 15 00:00:00 EDT 2022

COL6A6 inhibits the proliferation and metastasis of non-small cell lung cancer through the JAK signalling pathway.

Translational cancer research

Qiao H,Feng Y,Tang H

PA5-60958 was used in Western Blot to investigate the relationship between collagen type VI alpha 6 chain (COL6A6) and non-small cell lung cancer.

Fri Oct 01 00:00:00 EDT 2021

COL6A6 inhibits the proliferation and metastasis of non-small cell lung cancer through the JAK signalling pathway.

Translational cancer research

Qiao H,Feng Y,Tang H

PA5-60958 was used in Western Blot to investigate the relationship between collagen type VI alpha 6 chain (COL6A6) and non-small cell lung cancer.

Fri Oct 01 00:00:00 EDT 2021

Cancer-Associated Fibroblast Subgroups Showing Differential Promoting Effect on HNSCC Progression.

Cancers

Kang SH,Oh SY,Lee HJ,Kwon TG,Kim JW,Lee ST,Choi SY,Hong SH

Published figure using COL6A6 polyclonal antibody (Product # PA5-60958) in Immunohistochemistry

Sat Feb 06 00:00:00 EST 2021

COL6A6 interacted with P4HA3 to suppress the growth and metastasis of pituitary adenoma via blocking PI3K-Akt pathway.

Aging

Long R,Liu Z,Li J,Yu H

Published figure using COL6A6 polyclonal antibody (Product # PA5-60958) in Western Blot

Thu Oct 17 00:00:00 EDT 2019

生物信息学

蛋白别名: Collagen alpha-6(VI) chain; collagen, type VI, alpha 6

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基因别名: COL6A6

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UniProt ID: (Human) A6NMZ7

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Entrez Gene ID: (Human) 131873

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extracellular matrix structural protein

参与通路

cell adhesion collagen catabolic process