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Invitrogen
CRBN Polyclonal Antibody
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
图: 1 / 9
CRBN Antibody (PA5-38037) in ICC/IF
Immunofluorescence analysis of Protein cereblon was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CRBN Polyclonal Antibody (Product # PA5-38037) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790)... View More
产品信息
PA5-38037
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
16 amino acid peptide near the carboxy terminus of human CRBN
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
0.02% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2554641
产品详细信息
A recommended positive control is human testis tissue lysate.
At least two isoforms of CRBN are known to exist; this antibody will detect both isoforms.
靶标信息
CRBN, a member of the Lon protease protein family, plays a role in brain development. It is a component of the DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8. CRBN is thought to regulate the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. It is widely expressed and highly expressed in brain.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DKFZp781K0715; MGC27358; novel lethal; Protein cereblon; Protein PiL; protein x 0001
基因别名: 2610203G15Rik; 2900045O07Rik; AD-006; AF229032; AW108261; CRBN; MRT2; MRT2A; piL
UniProt ID: (Human) Q96SW2, (Mouse) Q8C7D2, (Rat) Q56AP7
Entrez Gene ID: (Human) 51185, (Mouse) 58799, (Rat) 297498
