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CRP Antibody (500-P242BT-1MG) in WB
Western Blot: To detect Human CRP by Western Blot analysis CRP Polyclonal Antibody, Biotin (Product # 500-P242BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human CRP is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P242BT-1MG
产品规格
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived, 23.2 kDa Recombinant Human CRP
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
保存条件
-20°C
运输条件
Ambient
RRID
AB_2930010
产品详细信息
AA Sequence of recombinant protein: discontinued.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human CRP. Anti-Human CRP-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human CRP by sandwich ELISA (using 100 µL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human CRP (500-P242) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human CRP.
Western Blot: To detect Human CRP by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human CRP is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P242BT-1MG will be provided as 2 x 500 µg
靶标信息
CRP (C-reactive protein) is a major cyclic, pentameric acute phase protein compound consisting of five identical, noncovalently bound, nonglycosylated subunits. Eeach subunit is made up of 206 amino acids, and has a molecular weight of 24 kDa. CRP is produced by the liver, and its plasma levels rise dramatically during inflammatory processes occurring in the body. CRP is an initiator of classical complement cascade, binds to several nuclear components (chromatin, histones, etc.), plays an important role in innate immunity, and mediates activities associated with pre-immune nonspecific host resistance. Specifically, there is a 100-1000 fold increase of CRP in response to injury, infection or inflammation. CRP is opsonic, an initiator of the classical complement cascade, and an activator of monocytes/macrophages. CRP also binds to several nuclear components including chromatin, histones and snRNP, suggesting that it may play a role as a scavenger during cell necrosis. CRP is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host, and initiates pathogen elimination by interacting with humoral and cellular effector systems in the blood. Patients with elevated basal levels of CRP have been shown to be an increased risk for hypertension and cardiovascular disease.
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