Invitrogen

CX3CR1 Monoclonal Antibody (2A9-1), Biotin, eBioscience™

CX3CR1 Antibody in Flow Cytometry (Flow)

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CX3CR1 Antibody (13-6099-82) in Flow

Staining of normal human peripheral blood cells with 0.125 µg of Rat IgG2b Isotype Control Biotin (Product # 13-4031-82) (left) or 0.125 µg of Anti-Human CX3CR1 Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Samples were subsequently stained intracellularly with Anti-Human Perforin FITC (Product # 11-9994-42). Cells were gated on CD16+CD56+ using Anti-Human CD16 eFluor® 450 (Product # 48-0168-42) and Anti-Human CD56 APC.

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产品信息

13-6099-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

2A9-1

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1963592

产品详细信息

Description: This 2A9-1 monoclonal antibody reacts with human CX3CR1, which is the receptor for fractalkine, a transmembrane chemokine of the CX3C family. CX3CR1 is a seven transmembrane G protein-coupled receptor expressed on CD16+ NK cells, T cells (e.g. CD8+, CD4+, and gamma/delta), and monocytes. In non-immune cells, CX3CR1 has been found on osteoclast precursors and microglia. Little to no CX3CR1 surface expression can be detected on B cells and granulocytes. Together, fractalkine and its receptor mediate cell-cell adhesion and chemotaxis of NK cells, T cells, and monocytes. The expression of CX3CR1 has also been correlated with high levels of intracellular perforin and granzyme B.

Applications Reported: This 2A9-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2A9-1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human, mouse, and rat CX3CR1 were cloned and designated V28 and CMKBRL1, CX3CR1, and RBS11, respectively. The encoded seven transmembrane protein was recently identified as the receptor for a novel transmembrane molecule, fractalkine, and renamed CX3CR1. Recently, CX3CR1 was found to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine. CX3CR1 mediates leukocyte migration an d adhesion. CX3CR1 is expressed in a variety of human tissues and cell lines.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Distinct gene expression patterns for CD14++ and CD16++ monocytes in preeclampsia.

Scientific reports

Vishnyakova P,Kuznetsova M,Poltavets A,Fomina M,Kiseleva V,Muminova K,Potapova A,Khodzhaeva Z,Pyregov A,Trofimov D,Elchaninov A,Sukhikh G,Fatkhudinov T

Published figure using CX3CR1 monoclonal antibody (Product # 13-6099-82) in Flow Cytometry

Wed Sep 14 00:00:00 EDT 2022

Microglia innately develop within cerebral organoids.

Nature communications

Ormel PR,Vieira de Sá R,van Bodegraven EJ,Karst H,Harschnitz O,Sneeboer MAM,Johansen LE,van Dijk RE,Scheefhals N,Berdenis van Berlekom A,Ribes Martínez E,Kling S,MacGillavry HD,van den Berg LH,Kahn RS,Hol EM,de Witte LD,Pasterkamp RJ

Published figure using CX3CR1 monoclonal antibody (Product # 13-6099-82) in Flow Cytometry

Tue Oct 09 00:00:00 EDT 2018

Modulation of Hematopoietic Lineage Specification Impacts TREM2 Expression in Microglia-Like Cells Derived From Human Stem Cells.

ASN neuro

Amos PJ,Fung S,Case A,Kifelew J,Osnis L,Smith CL,Green K,Naydenov A,Aloi M,Hubbard JJ,Ramakrishnan A,Garden GA,Jayadev S

Published figure using CX3CR1 monoclonal antibody (Product # 13-6099-82) in Flow Cytometry

Fri Dec 08 00:00:00 EST 2017

Pivotal Role for CD16+ Monocytes in Immune Surveillance of the Central Nervous System.

Journal of immunology (Baltimore, Md. : 1950)

Waschbisch A,Schröder S,Schraudner D,Sammet L,Weksler B,Melms A,Pfeifenbring S,Stadelmann C,Schwab S,Linker RA

13609982 was used in flow cytometry to analyze the frequency and phenotype of monocyte subpopulations in peripheral blood, cerebrospinal fluid, and brain biopsy material derived from multiple sclerosis patients

Mon Feb 15 00:00:00 EST 2016

The CD14+CD16+ inflammatory monocyte subset displays increased mitochondrial activity and effector function during acute Plasmodium vivax malaria.

PLoS pathogens

Antonelli LR,Leoratti FM,Costa PA,Rocha BC,Diniz SQ,Tada MS,Pereira DB,Teixeira-Carvalho A,Golenbock DT,Gonçalves R,Gazzinelli RT

13-6099 was used in Flow cytometry/Cell sorting to study inflammatory monocyte subsets displaying increased mitochondrial activity and effector function during malaria.

Mon Sep 01 00:00:00 EDT 2014

生物信息学

蛋白别名: Beta chemokine receptor-like 1; C-X3-C CKR-1; chemokine (C-C) receptor-like 1; chemokine (C-X3-C motif) receptor 1; chemokine (C-X3-C) receptor 1; CMK-BRL-1; CMK-BRL1; Cx3c; CX3C chemokine receptor 1; Fractalkine receptor; G protein-coupled receptor 13; G-protein coupled receptor 13; Scyd1; V28

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基因别名: CCRL1; CMKBRL1; CMKDR1; CX3CR1; GPR13; GPRV28; V28

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UniProt ID: (Human) P49238

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Entrez Gene ID: (Human) 1524

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功能

chemokine receptor activity protein binding C-X3-C chemokine receptor activity C-X3-C chemokine binding

参与通路