Invitrogen

CX3CR1 Monoclonal Antibody (2A9-1), PerCP-eFluor™ 710, eBioscience™

CX3CR1 Antibody in Flow Cytometry (Flow)

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CX3CR1 Antibody (46-6099-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) APC (Product # 17-0567-42) and Rat IgG2b K Isotype Control PerCP-eFluor® 710 (Product # 46-4031-82) (left) or Anti-Human CX3CR1 PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

46-6099-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

2A9-1

偶联物

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

激发/发射光谱

482/708 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10717390

产品详细信息

Description: This 2A9-1 monoclonal antibody reacts with human CX3CR1, which is the receptor for fractalkine, a transmembrane chemokine of the CX3C family. CX3CR1 is a seven transmembrane G protein-coupled receptor expressed on CD16+ NK cells, T cells (e.g. CD8+, CD4+, and gamma/delta), and monocytes. In non-immune cells, CX3CR1 has been found on osteoclast precursors and microglia. Little to no CX3CR1 surface expression can be detected on B cells and granulocytes. Together, fractalkine and its receptor mediate cell-cell adhesion and chemotaxis of NK cells, T cells, and monocytes. The expression of CX3CR1 has also been correlated with high levels of intracellular perforin and granzyme B.

Applications Reported: This 2A9-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2A9-1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human, mouse, and rat CX3CR1 were cloned and designated V28 and CMKBRL1, CX3CR1, and RBS11, respectively. The encoded seven transmembrane protein was recently identified as the receptor for a novel transmembrane molecule, fractalkine, and renamed CX3CR1. Recently, CX3CR1 was found to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine. CX3CR1 mediates leukocyte migration an d adhesion. CX3CR1 is expressed in a variety of human tissues and cell lines.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Distinct gene expression patterns for CD14++ and CD16++ monocytes in preeclampsia.

Scientific reports

Vishnyakova P,Kuznetsova M,Poltavets A,Fomina M,Kiseleva V,Muminova K,Potapova A,Khodzhaeva Z,Pyregov A,Trofimov D,Elchaninov A,Sukhikh G,Fatkhudinov T

Published figure using CX3CR1 monoclonal antibody (Product # 46-6099-42) in Flow Cytometry

Wed Sep 14 00:00:00 EDT 2022

Microglia innately develop within cerebral organoids.

Nature communications

Ormel PR,Vieira de Sá R,van Bodegraven EJ,Karst H,Harschnitz O,Sneeboer MAM,Johansen LE,van Dijk RE,Scheefhals N,Berdenis van Berlekom A,Ribes Martínez E,Kling S,MacGillavry HD,van den Berg LH,Kahn RS,Hol EM,de Witte LD,Pasterkamp RJ

Published figure using CX3CR1 monoclonal antibody (Product # 46-6099-42) in Flow Cytometry

Tue Oct 09 00:00:00 EDT 2018

Modulation of Hematopoietic Lineage Specification Impacts TREM2 Expression in Microglia-Like Cells Derived From Human Stem Cells.

ASN neuro

Amos PJ,Fung S,Case A,Kifelew J,Osnis L,Smith CL,Green K,Naydenov A,Aloi M,Hubbard JJ,Ramakrishnan A,Garden GA,Jayadev S

Published figure using CX3CR1 monoclonal antibody (Product # 46-6099-42) in Flow Cytometry

Fri Dec 08 00:00:00 EST 2017

Atezolizumab in combination with bevacizumab enhances antigen-specific T-cell migration in metastatic renal cell carcinoma.

Nature communications

Wallin JJ,Bendell JC,Funke R,Sznol M,Korski K,Jones S,Hernandez G,Mier J,He X,Hodi FS,Denker M,Leveque V,Cañamero M,Babitski G,Koeppen H,Ziai J,Sharma N,Gaire F,Chen DS,Waterkamp D,Hegde PS,McDermott DF

46-6099 was used in Flow cytometry/Cell sorting to suggest that the anti-VEGF and anti-PD-L1 combination improves antigen-specific T-cell migration.

Tue Aug 30 00:00:00 EDT 2016

生物信息学

蛋白别名: Beta chemokine receptor-like 1; C-X3-C CKR-1; chemokine (C-C) receptor-like 1; chemokine (C-X3-C motif) receptor 1; chemokine (C-X3-C) receptor 1; CMK-BRL-1; CMK-BRL1; Cx3c; CX3C chemokine receptor 1; Fractalkine receptor; G protein-coupled receptor 13; G-protein coupled receptor 13; Scyd1; V28

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基因别名: CCRL1; CMKBRL1; CMKDR1; CX3CR1; GPR13; GPRV28; V28

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UniProt ID: (Human) P49238

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Entrez Gene ID: (Human) 1524

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功能

chemokine receptor activity protein binding C-X3-C chemokine receptor activity C-X3-C chemokine binding

参与通路