Invitrogen

CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, Biotin, PeproTech®

CXCL12 alpha (SDF-1 alpha) Antibody in Western Blot (WB)

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CXCL12 alpha (SDF-1 alpha) Antibody (500-P87ABT-25UG) in WB

Western Blot: To detect Human SDF-1alpha by Western Blot analysis CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, Biotin (Product # 500-P87ABT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human SDF-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

CXCL12 alpha (SDF-1 alpha) Antibody in ELISA (ELISA)

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产品信息

500-P87ABT-25UG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

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酶联免疫吸附实验 (ELISA)

0.25-1.0 µg/mL

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产品规格

种属反应

Human

已发表种属

Bovine, Human

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived, 8.0 kDa Recombinant Human SDF-1alpha (CXCL12)

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偶联物

Biotin Biotin Biotin

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

RRID

AB_2930825

产品详细信息

AA Sequence of recombinant protein: KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human SDF-1alpha (CXCL12). Anti-Human SDF-1alpha (CXCL12)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Human SDF-1alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human SDF-1alpha (500-P87A) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human SDF-1alpha.

Western Blot: To detect Human SDF-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human SDF-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P87ABT-1MG will be provided as 2 x 500 µg

靶标信息

CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Carcinomas assemble a filamentous CXCL12-keratin-19 coating that suppresses T cell-mediated immune attack.

Proceedings of the National Academy of Sciences of the United States of America

Wang Z,Moresco P,Yan R,Li J,Gao Y,Biasci D,Yao M,Pearson J,Hechtman JF,Janowitz T,Zaidi RM,Weiss MJ,Fearon DT

500-P87ABt was used in Western Blotting to conclude that carcinomas assemble a CXCL12-KRT19 coating to evade cancer immune attack.

Tue Jan 25 00:00:00 EST 2022

Carcinomas assemble a filamentous CXCL12-keratin-19 coating that suppresses T cell-mediated immune attack.

Proceedings of the National Academy of Sciences of the United States of America

Wang Z,Moresco P,Yan R,Li J,Gao Y,Biasci D,Yao M,Pearson J,Hechtman JF,Janowitz T,Zaidi RM,Weiss MJ,Fearon DT

500-P87ABt was used in Western Blotting to conclude that carcinomas assemble a CXCL12-KRT19 coating to evade cancer immune attack.

Tue Jan 25 00:00:00 EST 2022

Chemokine axes in breast cancer: factors of the tumor microenvironment reshape the CCR7-driven metastatic spread of luminal-A breast tumors.

Journal of leukocyte biology

Weitzenfeld P,Kossover O,Körner C,Meshel T,Wiemann S,Seliktar D,Legler DF,Ben-Baruch A

500-P87ABt was used in Enzyme-linked immunosorbent assay to study the importance of chemokine axes in regulating the metastatic spread of breast cancer.

Wed Jun 01 00:00:00 EDT 2016

The chemokine CXCL12 regulates monocyte-macrophage differentiation and RUNX3 expression.

Blood

Sánchez-Martín L,Estecha A,Samaniego R,Sánchez-Ramón S,Vega MÁ,Sánchez-Mateos P

Thu Jan 06 00:00:00 EST 2011

生物信息学

蛋白别名: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 12; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 12; CXC; CXC motif chemokine; CXCL; H-SDF-1-alpha; Intercrine reduced in hepatomas; IRH; PBSF; Pre-B cell growth-stimulating factor; SDF 1 a; SDF 1 alpha; SDF 1α; SDF-1; SDF1 alpha; SDF1α; Stromal cell-derived factor 1; stromal cell-derived factor-1 alpha

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基因别名: CXCL12; IRH; PBSF; SCYB12; SDF1; SDF1A; SDF1B; TLSF; TPAR1

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UniProt ID: (Human) Q5IT36

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Entrez Gene ID: (Human) 6387

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功能

receptor binding chemokine activity growth factor activity chemoattractant activity chemokine receptor binding CXCR chemokine receptor binding

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