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AA Sequence of recombinant protein: VVVASELRCQ CLTTLPRVDF KNIQSLTVTP PGPHCAQTEV IATLKDGHEV CLNPEAPLVQ RIVQKILNKG KAN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat GRO-beta/MIP-2 (CXCL2). Anti-Rat GRO-beta/MIP-2 (CXCL2)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Rat GRO-beta/MIP-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat GRO-beta/MIP-2 (500-P75) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat GRO-beta/MIP-2.
Western Blot: To detect Rat GRO-beta/MIP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat GRO-beta/MIP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P75BT-1MG will be provided as 2 x 500 µg
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。