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Invitrogen
CYP3A4 Recombinant Rabbit Monoclonal Antibody (JA11-33)
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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CYP3A4 Antibody (MA5-32718) in ICC/IF
Immunofluorescence analysis of CYP3A4 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CYP3A4 Recombinant Rabbit Monoclonal Antibody (JA11-33) (Product # MA5-32718) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plu... View More
产品信息
MA5-32718
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JA11-33
抗原
Synthetic peptide within Human Cytochrome P450 3A4 aa 454-503
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2809995
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 1,4-cineole 2-exo-monooxygenase; 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; Albendazole monooxygenase (sulfoxide-forming); Albendazole sulfoxidase; Cholesterol 25-hydroxylase; CYP; CYPIIIA3; CYPIIIA4; Cytochrome P450 3A3; Cytochrome P450 3A4; Cytochrome P450 HLp; Cytochrome P450 NF-25; cytochrome P450, family 3, subfamily A, polypeptide 4; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4; Cytochrome P450-PCN1; glucocorticoid-inducible P450; MGC126680; Nifedipine oxidase; P450; P450-III, steroid inducible; P450-PCN1; Quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
基因别名: CP33; CP34; CYP3A; CYP3A3; CYP3A4; CYPIIIA3; CYPIIIA4; HLP; NF-25; P450C3; P450PCN1
UniProt ID: (Human) P08684
Entrez Gene ID: (Human) 1576
monooxygenase activity
steroid binding
iron ion binding
steroid hydroxylase activity
oxidoreductase activity
oxygen binding
enzyme binding
heme binding
vitamin D3 25-hydroxylase activity
taurochenodeoxycholate 6alpha-hydroxylase activity
caffeine oxidase activity
albendazole monooxygenase activity
quinine 3-monooxygenase activity
testosterone 6-beta-hydroxylase activity
aromatase activity
vitamin D 24-hydroxylase activity
oxygenase
lipid metabolic process
steroid catabolic process
xenobiotic metabolic process
steroid metabolic process
androgen metabolic process
alkaloid catabolic process
monoterpenoid metabolic process
drug metabolic process
calcitriol biosynthetic process from calciol
vitamin D metabolic process
drug catabolic process
exogenous drug catabolic process
heterocycle metabolic process
oxidation-reduction process
oxidative demethylation
