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CYP7A1 Antibody (PA5-119862) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of CYP7A1 in SiHa cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with CYP7A1 Polyclonal Antibody (Product # PA5-119862) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).
产品信息
PA5-119862
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein within human CYP7A1 aa 50-250
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2913434
产品详细信息
Positive Control: HepG2 cell lysate, Hela cell lysate, 293T cell lysate, SiHa, Hela.
Subcellular Location: Endoplasmic reticulum membrane, Microsome membrane.
靶标信息
CYP7A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 24-hydroxycholesterol 7-alpha-hydroxylase; Cholesterol 7-alpha-hydroxylase; Cholesterol 7-alpha-monooxygenase; CYPVII; Cytochrome P450 7A1; cytochrome P450, family 7, subfamily A, polypeptide 1; cytochrome P450, subfamily VIIA polypeptide 1
基因别名: CP7A; CYP7; CYP7A1; CYPVII
UniProt ID: (Human) P22680
Entrez Gene ID: (Human) 1581
