Invitrogen

CYP8B1 Polyclonal Antibody

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CYP8B1 Antibody (PA5-37088) in WB

Western blot analysis of CYP8B1 in Lane 1: HEK293T cell lysate, Lane 2: sp2/0 cell lysate, Lane 3: PC12 cell lysate. Samples were incubated with CYP8B1 polyclonal antibody (Product # PA5-37088) at a dilution of 1:500.

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产品信息

PA5-37088

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

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免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:200

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to amino acids 416-461 of human CYP8B1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 50% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2553905

产品详细信息

This antibody detects endogenous protein at a molecular weight of 58 kDa.

Purity is >95% by SDS-PAGE.

靶标信息

CYP8B1 (sterol 12-alpha-hydroxylase) is a member of the cytochrome P450 superfamily of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds. CYP8B1 is highly expressed in liver and is an important enzyme for bile acid synthesis. Specifically, CYP8B1 moderates the ratio of cholic acid over chenodeoxycholic acid to control the solubility of cholesterol. The gene encoding human CYP8B1 maps to chromosome 3p22.1. The CYP8B1 gene encodes a 501-amino acid protein and does not contain any introns. The CYP8B1 gene promoter is transactivated by hepatocyte nuclear factor 4a. In mice, disruption of the CYP8B1 gene prevents the synthesis of cholate, which is a primary bile acid.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Akkermansia muciniphila protects mice against an emerging tick-borne viral pathogen.

Nature microbiology

Xie J,Li H,Zhang X,Yang T,Yue M,Zhang Y,Chen S,Cui N,Yuan C,Li J,Zhu SJ,Liu W

PA5-37088 was used in Immunohistochemistry (Paraffin), Western Blot to indicate the probiotic potential of A. muciniphila in mitigating SFTSV infection.

Sun Jan 01 00:00:00 EST 2023

The metabolic genomic atlas reveals potential drivers and clinically relevant insights into the etiology of esophageal squamous cell carcinoma.

Theranostics

Liu X,Hong R,Du P,Yang D,He M,Wu Q,Li L,Wang Y,Chen J,Min Q,Li J,Zhang W,Zhan Q

PA5-37088 was used in Immunohistochemistry and Western Blotting to provide a deep insight into the metabolic landscape in Esophageal squamous cell carcinoma (ESCC), extending our understanding of the metabolic reconfiguration underlying the genomic basis of ESCC.

Thu Sep 29 00:00:00 EDT 2022

The metabolic genomic atlas reveals potential drivers and clinically relevant insights into the etiology of esophageal squamous cell carcinoma.

Theranostics

Liu X,Hong R,Du P,Yang D,He M,Wu Q,Li L,Wang Y,Chen J,Min Q,Li J,Zhang W,Zhan Q

Thu Sep 29 00:00:00 EDT 2022

Multidrug Resistance-Associated Protein 2 Deficiency Aggravates Estrogen-Induced Impairment of Bile Acid Metabolomics in Rats.

Frontiers in physiology

Alaei Faradonbeh F,Lastuvkova H,Cermanova J,Hroch M,Nova Z,Uher M,Hirsova P,Pavek P,Micuda S

PA5-37088 was used in Immunohistochemistry (Paraffin), Western Blot to demonstrate that Mrp2-deficient organism is more sensitive to estrogen-induced cholestasis.

Sat Jul 16 00:00:00 EDT 2022

Hepatocyte activity of the cholesterol sensor smoothened regulates cholesterol and bile acid homeostasis in mice.

iScience

Dalton GD,Oh SH,Tang L,Zhang S,Brown AL,Varadharajan V,Baleanu-Gogonea C,Gogonea V,Pathak P,Brown JM,Diehl AM

PA5-37088 was used in Immunohistochemistry (Paraffin), Western Blot to demonstrate G-protein-coupled receptor Smoothened in hepatocytes plays a regulatory role in sensing and feedback regulation of cholesterol balance driven by excess dietary cholesterol.

Fri Sep 24 00:00:00 EDT 2021

Glucocorticoids regulate AKR1D1 activity in human liver in vitro and in vivo.

The Journal of endocrinology

Nikolaou N,Arvaniti A,Appanna N,Sharp A,Hughes BA,Digweed D,Whitaker MJ,Ross R,Arlt W,Penning TM,Morris K,George S,Keevil BG,Hodson L,Gathercole LL,Tomlinson JW

PA5-37088 was used in Western Blotting to examine the impact of glucocorticoids on Steroid 5β-reductase (AKR1D1) activity in human liver HepG2 and Huh7 cells.

Fri May 01 00:00:00 EDT 2020

Glucocorticoids regulate AKR1D1 activity in human liver in vitro and in vivo.

The Journal of endocrinology

Nikolaou N,Arvaniti A,Appanna N,Sharp A,Hughes BA,Digweed D,Whitaker MJ,Ross R,Arlt W,Penning TM,Morris K,George S,Keevil BG,Hodson L,Gathercole LL,Tomlinson JW

PA5-37088 was used in Western Blotting to examine the impact of glucocorticoids on Steroid 5β-reductase (AKR1D1) activity in human liver HepG2 and Huh7 cells.

Fri May 01 00:00:00 EDT 2020

生物信息学

蛋白别名: 7 alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase; CYPVIIIB1; Cytochrome P450 8B1; cytochrome P450 8b1 sterol 12 alpha-hydrolase; cytochrome P450, 8b1, sterol 12 alpha-hydrolase; cytochrome P450, family 8, subfamily B, polypeptide 1; cytochrome P450, subfamily VIIIB (sterol 12-alpha-hydroxylase), polypeptide 1; sterol 12-alpha-hydrolase; Sterol 12-alpha-hydroxylase

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基因别名: CP8B; CYP12; CYP8B1

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UniProt ID: (Human) Q9UNU6, (Mouse) O88962

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Entrez Gene ID: (Human) 1582, (Mouse) 13124, (Rat) 81924

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功能

iron ion binding sterol 12-alpha-hydroxylase activity oxygen binding heme binding 7alpha-hydroxycholest-4-en-3-one 12alpha-hydroxylase activity monooxygenase activity oxidoreductase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen metal ion binding

参与通路

lipid metabolic process oxidation-reduction process bile acid biosynthetic process sterol metabolic process