Invitrogen

Calnexin Recombinant Rabbit Monoclonal Antibody (ARC0648)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他36个Calnexin抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (5)
高级验证 (1)

Calnexin Antibody in Immunocytochemistry (ICC/IF)

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Calnexin Antibody (MA5-35588) in ICC/IF

Immunofluorescence analysis of CANX was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Calnexin Recombinant Rabbit Monoclonal Antibody (ARC0648) (Product # MA5-35588) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa... View More

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产品信息

MA5-35588

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

ARC0648

抗原

A synthetic peptide corresponding to a sequence within amino acids 493-592 of human Calnexin (P27824)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.55 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2849488

产品详细信息

Immunogen sequence: LPVFLVILFC CSGKKQTSGM EYKKTDAPQP DVKEEEEEKE EEKDKGDEEE EGEEKLEEKQ KSDAEEDGGT VSQEEEDRKP KAEEDEILNR SPRNRKPRRE

靶标信息

Calnexin, also referred to as IP90, p88 and p90, is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatibility complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).

仅用于科研。不用于诊断过程。未经明确授权不得转售。