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Calpain 1 Antibody (PA5-87524) in WB
Western Blot analysis of Calpain 1 in extracts of various cell lines using Calpain 1 Polyclonal Antibody (Product # PA5-87524) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
产品信息
PA5-87524
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 20-330 of human CAPN1
偶联物
Unconjugated
形式
Liquid
浓度
1.04 mg/mL
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2804223
产品详细信息
Immunogen sequence: KQRARELGLG RHENAIKYLG QDYEQLRVRC LQSGTLFRDE AFPPVPQSLG YKDLGPNSSK TYGIKWKRPT ELLSNPQFIV DGATRTDICQ GALGDCWLLA AIASLTLNDT LLHRVVPHGQ SFQNGYAGIF HFQLWQFGEW VDVVVDDLLP IKDGKLVFVH SAEGNEFWSA LLEKAYAKVN GSYEALSGGS TSEGFEDFTG GVTEWYELRK APSDLYQIIL KALERGSLLG CSIDISSVLD MEAITFKKLV KGHAYSVTGA KQVNYRGQVV SLIRMRNPWG EVEWTGAWSD SSSEWNNVDP YERDQLRVKM E; Positive Samples: SW480, A-549, BT-474, MCF-7, Jurkat; Cellular Location: Cell membrane, Cytoplasm
靶标信息
The calpains including Calpain 1 (CAPN1), calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. Diseases associated with CAPN1 include Spastic Paraplegia 76, Autosomal Recessive and Spasticity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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