Invitrogen

Cas9 Monoclonal Antibody (7A9-3A3)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

7 篇参考文献

查看其他26个Cas9抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (3)
高级验证 (2)

Cas9 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 5

Cas9 Antibody (MA1-201) in ICC/IF

Immunofluorescent analysis of Cas9 (green) in U2OS cells (left panel) and stable Cas9 expressing U2OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Cas9 monoclonal antibody (Product # MA1-201) at a dilution of 5 µg/mL in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG Superclonal™ Secondary Antibody, Ale... View More

Product Image Gallery

Cas9 Antibody — The specificity of anti-Cas9 monoclonal antibody (Product # MA1-201) was demonstrated by immunofluorescent detection of Cas9 expression in stable expressing Cas9 U2OS cells (right panel), but not in wild type U2OS cell lysate (left panel) which does not express Cas9. {RE}

产品信息

MA1-201

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

查看6篇已发表文章 6篇已发表文章

免疫细胞化学 (ICC/IF)

1:200 - 1:500

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Bacteria

已发表种属

Bacteria, Mouse, Virus

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

7A9-3A3

抗原

Cas9 nuclease

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS with 1mg/mL BSA, 30% glycerol

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2610640

产品详细信息

Cas9 (7A9-3A3) Mouse mAb recognizes transfected levels of total Cas9 protein.

Product was tested in Streptococcus pyogenes bacteria.

靶标信息

Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering. The system is unique and flexible due to its dependence on RNA as the element that targets the nuclease to a desired DNA sequence. It can be used to induce indel mutations, specific sequence replacements/insertions, and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9). SaCas9 has a number of properties that make it advantageous for genome editing, including its small size, high efficiency, nickase activity, and apparent specificity. SaCas9 principally recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9 with an ideal spacer lengths of 24 to 20 nucleotides. In comparison to SpCas9, SaCas9 is smaller (1368 AA versus 1053 AA) and generates indels at a comparable rate when directed against target DNA with the mutually permissive NGGRRT PAM. This makes it the preferred enzyme for all-in-one delivery of Cas9 and multiple gRNA expression cassettes with Adeno-associated vectors.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Gene Editing in Human Induced Pluripotent Stem Cells Using Doxycycline-Inducible CRISPR-Cas9 System.

Methods in molecular biology (Clifton, N.J.)

Thamodaran V,Rani S,Velayudhan SR

MA1-201 was used in Western Blotting to describe a stepwise protocol for the generation and characterization of such an iPSC line (AAVS1-PDi-Cas9 iPSC) with a doxycycline (dox)-inducible Cas9 expression cassette from the AAVS1 safe harbor site and efficient editing of target genes with lentiviral vectors expressing gRNAs.

Thu Jun 23 00:00:00 EDT 2022

Small-molecule inhibitors of histone deacetylase improve CRISPR-based adenine base editing.

Nucleic acids research

Shin HR,See JE,Kweon J,Kim HS,Sung GJ,Park S,Jang AH,Jang G,Choi KC,Kim I,Kim JS,Kim Y

MA1-201 was used in Western Blot to support the use of romidepsin as a viable option to improve base editing efficiency in biomedical research and therapeutic genome engineering.

Fri Feb 26 00:00:00 EST 2021

Adenine Base Editor Ribonucleoproteins Delivered by Lentivirus-Like Particles Show High On-Target Base Editing and Undetectable RNA Off-Target Activities.

The CRISPR journal

Lyu P,Lu Z,Cho SI,Yadav M,Yoo KW,Atala A,Kim JS,Lu B

MA1-201 was used in Western Blotting to describe our development of a delivery method to minimize ABEs' RNA off-target activity.

Mon Feb 01 00:00:00 EST 2021

Engineered extracellular vesicles as versatile ribonucleoprotein delivery vehicles for efficient and safe CRISPR genome editing.

Journal of extracellular vesicles

Yao X,Lyu P,Yoo K,Yadav MK,Singh R,Atala A,Lu B

MA1-201 was used in Western Blotting to show the aptamer and ABP interactions can be utilized to actively enrich RNPs into EVs for improved genome editing efficiency and safety.

Mon Mar 01 00:00:00 EST 2021

Restoration of visual function in adult mice with an inherited retinal disease via adenine base editing.

Nature biomedical engineering

Suh S,Choi EH,Leinonen H,Foik AT,Newby GA,Yeh WH,Dong Z,Kiser PD,Lyon DC,Liu DR,Palczewski K

MA1-201 was used in Western Blotting to show, in adult mice, that a subretinal injection of a lentivirus expressing an ABE and a single-guide RNA targeting a de novo nonsense mutation in the Rpe65 gene corrects the pathogenic mutation with up to 29% efficiency and with minimal formation of indel and off-target mutations, despite the absence of the canonical NGG sequence as a protospacer-adjacent motif.

Mon Feb 01 00:00:00 EST 2021

Primary effusion lymphoma enhancer connectome links super-enhancers to dependency factors.

Nature communications

Wang C,Zhang L,Ke L,Ding W,Jiang S,Li D,Narita Y,Hou I,Liang J,Li S,Xiao H,Gottwein E,Kaye KM,Teng M,Zhao B

MA1-201 was used in cell expression to provide insights into PEL molecular pathogenesis.

Wed Dec 09 00:00:00 EST 2020

Melanocyte Stem Cell Activation and Translocation Initiate Cutaneous Melanoma in Response to UV Exposure.

Cell stem cell

Moon H,Donahue LR,Choi E,Scumpia PO,Lowry WE,Grenier JK,Zhu J,White AC

MA1-201 was used in Western Blotting to define the role of melanocyte stem cells as melanoma cells of origin, and identify the extrinsic factors required for the critical early steps of melanoma initiation.

Thu Nov 02 00:00:00 EDT 2017

生物信息学

蛋白别名: cas9; CASP 9; Caspase-9 precursor; CASPASE-9c; Caspase9; CRISPR-Cas9; CRISPR9; CTD; OTTHUMP00000002324; RNCASP9; RP11-265F14.3; s aureus

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