Invitrogen

Caspase 8 Recombinant Rabbit Monoclonal Antibody (ARC0028)

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

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产品信息

MA5-35296

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:500-1:2,000
-
产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

ARC0028

抗原

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Caspase-8 (Q14790)
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.43 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2849198

产品详细信息

Immunogen sequence: EELCGVMTIS DSPREQDSES QTLDKVYQMK SKPRGYCLII NNHNFAKARE KVPKLHSIRD RNGTHLDAGA LTTTFEELHF EIKPHDDCTV EQIYEILKIY Q

靶标信息

Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases play a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a pro-domain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. Caspase 8 is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of Caspase 8 suggests that it may interact with Fas-interacting protein FADD. Caspase 8 was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Caspase 8 binds to the death effector domain (DED) of FADD through an analogous DED domain present in tandem in the pro-form of the Caspase 8 protein. Activated Caspase 8 then activates other downstream caspases including Caspase 9, thereby committing the cell to undergo apoptosis. In addition, Caspase 8 also reacts with Jurkat cells and Tonsil. Overexpression of Caspase 8 induces apoptosis, which can be blocked by inhibitors specific for the ICE family. Many alternatively spliced transcript variants encoding different isoforms have been described for Caspase 8, however, not all variants have had their full-length sequences determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; CASP-8; caspase 8, apoptosis-related cysteine peptidase; caspase 8, apoptosis-related cysteine protease; Caspase-8; Caspase-8 precursor; Caspase8; EC 3.4.22.61; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; FLICE; FLJ17672; ICE-like apoptotic protease 5; ICE8; MACH; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; MGC78473; MORT1-associated ced-3 homolog; OTTHUMP00000163720; OTTHUMP00000165063; OTTHUMP00000206557; OTTHUMP00000206581

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基因别名: ALPS2B; CAP4; Casp-8; CASP8; FLICE; MACH; MCH5

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UniProt ID: (Human) Q14790

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Entrez Gene ID: (Human) 841

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功能
cysteine-type endopeptidase activity death receptor binding tumor necrosis factor receptor binding protein binding peptidase activity cysteine-type peptidase activity ubiquitin protein ligase binding protein complex binding death effector domain binding identical protein binding scaffold protein binding cysteine-type endopeptidase activity involved in apoptotic process cysteine-type endopeptidase activity involved in apoptotic signaling pathway protease
参与通路
proteolysis apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process cellular component disassembly involved in execution phase of apoptosis cell surface receptor signaling pathway response to cold regulation of tumor necrosis factor-mediated signaling pathway regulation of necrotic cell death natural killer cell activation macrophage differentiation response to cobalt ion response to estradiol response to lipopolysaccharide response to tumor necrosis factor TRIF-dependent toll-like receptor signaling pathway TRAIL-activated apoptotic signaling pathway suppression by virus of host cysteine-type endopeptidase activity involved in apoptotic process T cell activation B cell activation positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of I-kappaB kinase/NF-kappaB signaling response to ethanol positive regulation of macrophage differentiation positive regulation of proteolysis response to antibiotic protein heterooligomerization proteolysis involved in cellular protein catabolic process syncytiotrophoblast cell differentiation involved in labyrinthine layer development nucleotide-binding oligomerization domain containing signaling pathway cellular response to mechanical stimulus cellular response to organic cyclic compound death-inducing signaling complex assembly apoptotic signaling pathway extrinsic apoptotic signaling pathway execution phase of apoptosis activation of cysteine-type endopeptidase activity activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
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