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Caspase 9 (Cleaved Asp330) Antibody (PA5-77889) in WB
Western Blot using Caspase 9 (Cleaved Asp330) Polyclonal Antibody (Product # PA5-77889). Untreated (–) and treated (+) Jurkat whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 9 (Cleaved Asp330) Polyclonal Antibody (Product # PA5-77889) diluted at 1:500.
产品信息
PA5-77889
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Caspase 9 (cleaved Asp330). The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
2.73 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2735576
产品详细信息
Positive Control: Jurkat (10 µM Etoposide treatment for 24 hr)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Caspase 9 (Cleaved Asp330) encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 25 kDa caspase-9 dominant negative protein; APAF-3; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; CASP-9; caspase 9, apoptosis-related cysteine peptidase; Caspase-9; caspase-9 dominant negative form; caspase-9-carboxyl-terminal divergent; ICE-LAP6; ICE-like apoptotic protease 6; protein phosphatase 1, regulatory subunit 56
基因别名: APAF-3; APAF3; Casp-9-CTD; CASP9; Casp9_v1; ICE-LAP6; MCH6; PPP1R56
UniProt ID: (Human) P55211
Entrez Gene ID: (Human) 842, (Rat) 58918
proteolysis
apoptotic process
cellular response to DNA damage stimulus
aging
intrinsic apoptotic signaling pathway in response to DNA damage
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
platelet formation
response to cobalt ion
response to estradiol
response to lipopolysaccharide
glial cell apoptotic process
cellular response to UV
signal transduction in response to DNA damage
positive regulation of neuron apoptotic process
response to antibiotic
cellular response to dexamethasone stimulus
extrinsic apoptotic signaling pathway in absence of ligand
regulation of response to DNA damage stimulus
activation of cysteine-type endopeptidase activity involved in apoptotic process
response to UV
response to organic cyclic compound
positive regulation of apoptotic process
cellular response to organic cyclic compound
