Rockland

Catalase Polyclonal Antibody

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Catalase Antibody (100-4151) in WB

Western Blot of Rabbit Anti-Catalase (Bovine Liver) Antibody. Lane 1: CHO K1 Lysate 10µg. Lane 2: CHO S Lysate 10µg. Lane 3: Opal Pre-stained Molecular Weight Marker (p/n MB-210-0500). Primary Antibody: Rabbit Anti-Catalase at 1:500 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG CY5 (p/n 611-110-122) at 1:10,000 for 30 in at RT. Expect: ~59.8kDa.

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产品信息

100-4151

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000-1:20,000

免疫组化 (IHC)

Assay-dependent

酶联免疫吸附实验 (ELISA)

1:100,000-1:1,000,000

免疫沉淀 (IP)

1:5,000-1:20,000

产品规格

种属反应

Bovine

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Anti-Catalase Antibody was produced by repeated immunizations with bovine liver catalase protein.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

85 mg/mL

保存液

0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

产品详细信息

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Catalase [Bovine Liver]. Cross reactivity against Catalase from other tissues and species may occur but has not been specifically determined.

靶标信息

Catalase is a homotetrameric heme-containing enzyme present within the matrix of all peroxisomes. It carries out a dismutation reaction in which hydrogen peroxide is converted to water and oxygen. Human catalase has the last four amino acids (-KANL) at the extreme C-terminus for peroxisome targeting. The monomer of human catalase is 61.3 kDa in molecular size. Catalase has been implicated as an important factor in inflammation, mutagenesis, prevention of apoptosis, and stimulation of a wide spectrum of tumors. Loss of catalase leads to the human genetic disease, acatalasemia, or Takahara's disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。