Invitrogen

Catalase Polyclonal Antibody

Catalase Antibody in Immunocytochemistry (ICC/IF)

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Catalase Antibody (PA5-29650) in ICC/IF

Immunofluorescence analysis of Catalase was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Catalase Polyclonal Antibody (Product # PA5-29650) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731)... View More

Catalase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-29650

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:10,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

5 µg/mL

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 89 and 341 of Human Catalase

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 10% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2547125

产品详细信息

Recommended positive controls: Molt-4, Raji, rat kidney, CAT-transfected 293T.

Predicted reactivity: Mouse (93%), Rat (92%), Zebrafish (82%), Dog (95%), Pig (94%), Chicken (87%), Bovine (93%), Guinea pig (91%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Catalase is a homotetrameric heme-containing enzyme present within the matrix of all peroxisomes. It carries out a dismutation reaction in which hydrogen peroxide is converted to water and oxygen. Human catalase has the last four amino acids (-KANL) at the extreme C-terminus for peroxisome targeting. The monomer of human catalase is 61.3 kDa in molecular size. Catalase has been implicated as an important factor in inflammation, mutagenesis, prevention of apoptosis, and stimulation of a wide spectrum of tumors. Loss of catalase leads to the human genetic disease, acatalasemia, or Takahara's disease.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。