Invitrogen

Cathepsin L Monoclonal Antibody (33-2), eBioscience™

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

3个已发表图片

6 篇参考文献

查看其他20个Cathepsin L抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

Cathepsin L Antibody in Western Blot (WB)

图: 1 / 10

Cathepsin L Antibody (BMS1032) in WB

Knockout of Cathepsin L was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR1042354_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of Cathepsin L was performed by loading 30 µg of A549 Cas9 (Lane 1), andA549 Cathepsin L KO (Lane 2) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlo... View More

Cathepsin L Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

产品信息

BMS1032

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

查看5篇已发表文章 5篇已发表文章

免疫组化（冰冻） (IHC (F))

Assay-Dependent

免疫细胞化学 (ICC/IF)

5 µg/mL

查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-Dependent

产品规格

种属反应

Human

已发表种属

Human, Non-human primate

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

33-2

抗原

Rec. Human Cathepsin

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2-7.4

内含物

no preservative

保存条件

-20°C

运输条件

Wet ice

RRID

AB_10596643

产品详细信息

Description: Cathepsin L is a lysosomal cysteine proteinase consisting of a heavy chain of about 25 kDa and a light chain of about 5 kDa derived proteolytically from the same precursor. Cathepsin L has been shown to be produced by many different cell types. Since cathepsin L is capable of degrading protein constituents of the extracellular matrix it is assumed to play a crucial role in tumor progression and metastasis and a number of other disorders where the destruction of the extracellular matrix is the major cause of disease (e.g. rheumatoid arthritis, neurodegeneration).

Applications Tested: ELISA, Immunohistochemistry (Frozen), Western Blotting.

靶标信息

The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

SARS-CoV-2 Enters Human Leydig Cells and Affects Testosterone Production In Vitro.

Cells

Li L,Sottas CM,Chen HY,Li Y,Cui H,Villano JS,Mankowski JL,Cannon PM,Papadopoulos V

BMS1032 was used in Western Blotting to show that SARS-CoV-2 can enter hLLCs through a distinct pathway and alter testosterone production.

Thu Apr 20 00:00:00 EDT 2023

Genome-scale CRISPR screens identify host factors that promote human coronavirus infection.

Genome medicine

Grodzki M,Bluhm AP,Schaefer M,Tagmount A,Russo M,Sobh A,Rafiee R,Vulpe CD,Karst SM,Norris MH

BMS1032 was used in Western Blot to substantiate and expand the growing body of literature focused on understanding key human coronavirus-host cell interactions and exploit that knowledge for rational antiviral drug development.

Thu Jan 27 00:00:00 EST 2022

Genome-scale CRISPR screens identify host factors that promote human coronavirus infection.

Genome medicine

Grodzki M,Bluhm AP,Schaefer M,Tagmount A,Russo M,Sobh A,Rafiee R,Vulpe CD,Karst SM,Norris MH

Thu Jan 27 00:00:00 EST 2022

SerpinB3 drives cancer stem cell survival in glioblastoma.

Cell reports

Lauko A,Volovetz J,Turaga SM,Bayik D,Silver DJ,Mitchell K,Mulkearns-Hubert EE,Watson DC,Desai K,Midha M,Hao J,McCortney K,Steffens A,Naik U,Ahluwalia MS,Bao S,Horbinski C,Yu JS,Lathia JD

Published figure using Cathepsin L monoclonal antibody (Product # BMS1032) in Immunocytochemistry

Tue Sep 13 00:00:00 EDT 2022

SARS-CoV-2 Spike triggers barrier dysfunction and vascular leak via integrins and TGF-β signaling.

Nature communications

Biering SB,Gomes de Sousa FT,Tjang LV,Pahmeier F,Zhu C,Ruan R,Blanc SF,Patel TS,Worthington CM,Glasner DR,Castillo-Rojas B,Servellita V,Lo NTN,Wong MP,Warnes CM,Sandoval DR,Clausen TM,Santos YA,Fox DM,Ortega V,Näär AM,Baric RS,Stanley SA,Aguilar HC,Esko JD,Chiu CY,Pak JE,Beatty PR,Harris E

BMS1032 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to offer mechanistic insight into SARS-CoV-2-triggered vascular leak, providing a starting point for development of therapies targeting COVID-19.

Fri Dec 09 00:00:00 EST 2022

TMPRSS2 expression dictates the entry route used by SARS-CoV-2 to infect host cells.

The EMBO journal

Koch J,Uckeley ZM,Doldan P,Stanifer M,Boulant S,Lozach PY

BMS1032 was used in Western Blot to indicate that SARS-CoV-2 infects cells through distinct, mutually exclusive entry routes and highlight the importance of TMPRSS2 for SARS-CoV-2 sorting into either pathway.