Invitrogen

Caveolin 2 Polyclonal Antibody

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

2 篇参考文献

查看其他17个Caveolin 2抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (3)
高级验证 (1)

Caveolin 2 Antibody in Immunocytochemistry (ICC/IF)

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Caveolin 2 Antibody (PA1-065) in ICC/IF

Immunofluorescence analysis of Caveolin2 was performed using 3T3L-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Caveolin 2 Polyclonal Antibody (Product # PA1-065) at 5µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat... View More

Caveolin 2 Antibody in Western Blot (WB)

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产品信息

PA1-065

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

5 µg/mL

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues M(1) G L E T E K A D V Q L F M A D D A Y(19) C of rat CAV2.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2244214

产品详细信息

PA1-065 detects caveolin-2 from rat and mouse tissues tissues. This antibody does not detect caveolin-1 or -3.

PA1-065 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a ~21 kDa protein representing caveolin-2 from rat heart homogenate.

The PA1-065 immunogen is a synthetic peptide corresponding to residues M(1) G L E T E K A D V Q L F M A D D A Y(19) C of rat CAV2. This sequence is completely conserved in mouse, and ~90% conserved in human caveolin-2. The PA1-065 immunizing peptide (Cat. # PEP-080) is available for use in neutralization and control experiments.

靶标信息

Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene family which are known to encode 21-24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Enhanced phosphoinositide 3-kinase(p110α) activity prevents diabetes-induced cardiomyopathy and superoxide generation in a mouse model of diabetes.

Diabetologia

Ritchie RH,Love JE,Huynh K,Bernardo BC,Henstridge DC,Kiriazis H,Tham YK,Sapra G,Qin C,Cemerlang N,Boey EJ,Jandeleit-Dahm K,Du XJ,McMullen JR

PA1-065 was used in Western Blotting to demonstrate that reduced phosphoinositide 3-kinase (PI3K) activity accelerates the development of diabetic cardiomyopathy.