Invitrogen

Cdc42 Recombinant Rabbit Monoclonal Antibody (JJ086-04)

查看其他13个CDC42抗体

抗体检测数据 (5)

Cdc42 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cdc42 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Cdc42 Antibody (MA5-32472) in IHC (P)

Immunohistochemical analysis of Cdc42 of paraffin-embedded Human breast carcinoma tissue using a Cdc42 Monoclonal antibody (Product #MA5-32472). Counter stained with hematoxylin.

Cdc42 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cdc42 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cdc42 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cdc42 Antibody in Western Blot (WB)

Cdc42 Antibody in Flow Cytometry (Flow)

Product Image Gallery

Cdc42 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Cdc42 of paraffin-embedded Human breast carcinoma tissue using a Cdc42 Monoclonal antibody (Product #MA5-32472). Counter stained with hematoxylin.

Cdc42 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Cdc42 of paraffin-embedded Human pancreas tissue using a Cdc42 Monoclonal antibody (Product #MA5-32472). Counter stained with hematoxylin.

Cdc42 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Cdc42 of paraffin-embedded Mouse pancreas tissue using a Cdc42 Monoclonal antibody (Product #MA5-32472). Counter stained with hematoxylin.

Cdc42 Antibody in Western Blot (WB) — Western blot analysis of Cdc42 in different lysates using a Monoclonal antibody (Product #MA5-32472) at a dilution of 1:1,000. Positive control: Lane 1: Hela, Lane 2: Jurkat, Lane 3: HepG2.

Cdc42 Antibody in Flow Cytometry (Flow) — Flow Cytometric analysis of Cdc42 in Hela cells using a Cdc42 Monoclonal Antibody (Product # MA5-32472) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

产品信息

MA5-32472

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

流式细胞分析 (Flow)

1:50-1:100

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JJ086-04

抗原

Recombinant protein within Human CDC42 aa 30-191

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2809749

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Rac1 is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for the Rac1 gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Cell division control protein 42 homolog; cell division cycle 42 (GTP binding protein); cell division cycle 42 homolog; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)); G25K GTP-binding protein; growth-regulating protein; GTP binding protein, 25kDa; RP1-224A6.5; small GTP binding protein CDC42

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基因别名: AI747189; AU018915; CDC42; CDC42Hs; G25K; TKS

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UniProt ID: (Human) P60953, (Rat) Q8CFN2, (Mouse) P60766

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Entrez Gene ID: (Human) 998, (Rat) 64465, (Mouse) 12540

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GTPase activity protein binding GTP binding protein kinase binding GTP-dependent protein binding mitogen-activated protein kinase kinase kinase binding thioesterase binding apolipoprotein A-I receptor binding identical protein binding Rho GDP-dissociation inhibitor binding ubiquitin protein ligase activity nucleotide binding small GTPase

参与通路

sprouting angiogenesis cardiac conduction system development keratinocyte development Golgi organization regulation of mitotic nuclear division nuclear migration establishment or maintenance of cell polarity small GTPase mediated signal transduction blood coagulation positive regulation of gene expression negative regulation of gene expression protein ubiquitination substantia nigra development actin cytoskeleton organization macrophage differentiation positive regulation of cell growth hair follicle morphogenesis positive regulation of pseudopodium assembly T cell costimulation negative regulation of protein complex assembly keratinization regulation of protein stability positive regulation of cytokinesis positive regulation of peptidyl-serine phosphorylation adherens junction organization cellular protein localization establishment or maintenance of apical/basal cell polarity multicellular organism growth dendritic cell migration Fc-gamma receptor signaling pathway involved in phagocytosis negative regulation of epidermal growth factor receptor signaling pathway regulation of protein catabolic process regulation of protein heterodimerization activity positive regulation of neuron apoptotic process positive regulation of phosphatidylinositol 3-kinase activity positive regulation of DNA replication regulation of protein kinase activity positive regulation of JNK cascade filopodium assembly vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway neuron fate determination actin filament bundle assembly regulation of small GTPase mediated signal transduction positive regulation of muscle cell differentiation regulation of filopodium assembly establishment of Golgi localization positive regulation of synapse structural plasticity regulation of attachment of spindle microtubules to kinetochore heart contraction canonical Wnt signaling pathway Wnt signaling pathway, planar cell polarity pathway positive regulation of epithelial cell proliferation involved in lung morphogenesis submandibular salivary gland formation epithelial-mesenchymal cell signaling hair follicle placode formation positive regulation of hair follicle cell proliferation organelle transport along microtubule actin filament branching epithelial cell-cell adhesion positive regulation of intracellular protein transport positive regulation of substrate adhesion-dependent cell spreading positive regulation of protein phosphorylation endocytosis actin filament organization metabolic process response to glucose single organismal cell-cell adhesion regulation of exocytosis establishment of cell polarity positive regulation of catalytic activity positive regulation of MAPK cascade epidermis morphogenesis regulation of protein metabolic process nucleus localization Rho protein signal transduction nervous system development endosomal transport cell differentiation