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Cdc6 Antibody (PA5-77901) in ICC/IF
Immunofluorescence analysis of CDC6 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Cdc6 Polyclonal Antibody (Product # PA5-77901) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:20... View More
产品信息
PA5-77901
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the center region of human CDC6. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
1.36 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2735620
产品详细信息
Positive Control: Jurkat, HeLa, HeLa (10 mM Hydroxyurea treatment for 18 hr), Neuro2A, GL261, C8D30, BCL-1, Raw264.7, C2C12, PC-12, Rat2
Predicted Reactivity: Bovine (89%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CDC18(S.pombe); Cdc18-related protein; CDC6 cell division cycle 6 homolog; CDC6-related protein; Cell division control protein 6 homolog; cell division cycle 6 homolog; HsCdc18; HsCDC6; p62(cdc6)
基因别名: CDC18L; CDC6; HsCDC18; HsCDC6
UniProt ID: (Human) Q99741, (Mouse) O89033
Entrez Gene ID: (Human) 990, (Mouse) 23834, (Rat) 360621
DNA replication checkpoint
regulation of cyclin-dependent protein serine/threonine kinase activity
G1/S transition of mitotic cell cycle
regulation of transcription involved in G1/S transition of mitotic cell cycle
DNA replication
DNA replication initiation
mitotic nuclear division
traversing start control point of mitotic cell cycle
negative regulation of DNA replication
negative regulation of cell proliferation
regulation of mitotic metaphase/anaphase transition
positive regulation of cytokinesis
positive regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of fibroblast proliferation
cell division
positive regulation of chromosome segregation
cellular response to vasopressin
cellular response to angiotensin
cell cycle
