Invitrogen

ChAT Recombinant Rabbit Monoclonal Antibody (8E7)

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ChAT Antibody in Immunocytochemistry (ICC/IF)

ChAT Antibody in Immunocytochemistry (ICC/IF)

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ChAT Antibody (MA5-50128) in ICC/IF

Immunocytochemistry analysis of SH-SY5Y with ChAT monoclonal antibody (Product # MA5-50128) at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 498-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

ChAT Antibody in Immunocytochemistry (ICC/IF)

ChAT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ChAT Antibody in Western Blot (WB)

ChAT Antibody in Flow Cytometry (Flow)

Product Image Gallery

ChAT Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of SH-SY5Y with ChAT monoclonal antibody (Product # MA5-50128) at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 498-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

ChAT Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis with a ChAT monoclonal antibody (Product # MA5-50128) using a dilution of 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.22% DAB.

ChAT Antibody in Western Blot (WB) — Western blot analysis of ChAT in Mouse Brain tissue lysate using a dilution of 1:500. Samples were incubated with ChAT monoclonal antibody (Product # MA5-50128). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 70 kDa. Observed band size: 70 kDa.

ChAT Antibody in Flow Cytometry (Flow) — Flow cytometry analysis overlay peak curve showing SH-SY5Y cells stained with ChAT monoclonal antibody (Product # MA5-50128) for 45 min at 4. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at (1x10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

产品信息

MA5-50128

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:50-1:200

-

流式细胞分析 (Flow)

1:50-1:200

-

酶联免疫吸附实验 (ELISA)

Assay-dependent

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

8E7

抗原

A synthesized peptide derived from Human CHAT

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.8 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C or -80°C if preferred

运输条件

Wet ice

RRID

AB_3093691

靶标信息

ChAT catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: acetyl CoA; acetyl CoA:choline O-acetyltransferase; CHOACTase; choline acetylase; Choline O-acetyltransferase

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基因别名: B230380D24Rik; CHAT; CHOACTASE; CMS1A; CMS1A2; CMS6

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UniProt ID: (Human) P28329

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Entrez Gene ID: (Human) 1103, (Mouse) 12647

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choline O-acetyltransferase activity choline binding transferase activity transferase activity, transferring acyl groups acyltransferase

参与通路

antral ovarian follicle growth response to hypoxia phosphatidylcholine biosynthetic process neurotransmitter secretion response to nutrient memory acetylcholine biosynthetic process response to drug response to ethanol chemical synaptic transmission neuromuscular synaptic transmission muscle organ development establishment of synaptic specificity at neuromuscular junction rhythmic behavior adult walking behavior dendrite development neuron differentiation neurotransmitter biosynthetic process rhythmic excitation