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Invitrogen
Chicken anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (A-21443) in ICC/IF
Immunofluorescence analysis of Chicken anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 (Product # A-21443) was performed using MCF 10A (positive model) and A-431 (negative model) in cells stained with Vimentin Polyclonal antibody (Product # PA5-27231). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 2% ... View More
产品信息
A-21443
应用
建议稀释比
免疫印迹 (WB)
1:5,000-1:10,000
免疫组化 (IHC)
1-10 µg/mL
免疫细胞化学 (ICC/IF)
1:500-1:2,000
产品规格
种属反应
Rabbit
宿主/亚型
Chicken
/ IgY
分类
Polyclonal
类型
Secondary Antibody
抗原
Gamma Immunoglobins Heavy and Light chains
偶联物
激发/发射光谱
650/671 nm
查看光谱
形式
Liquid
浓度
2 mg/mL
纯化类型
purified
保存液
PBS, pH 7.5
内含物
5mM sodium azide
保存条件
4° C, store in dark
运输条件
Ambient
RRID
AB_2535861
靶标
IgG
交叉吸附
Against human and mouse IgG prior to conjugation
抗体形式
Whole Antibody
产品详细信息
This chicken anti-rabbit antibody reacts with IgG heavy chains and all classes of immunoglobulin light chains from rabbit. Chicken secondary antibodies have gained popularity because they demonstrate a lower level of nonspecific binding. Chicken antibodies lack a classic “Fc” domain and will not bind to protein A or protein G, nor will they bind to mammalian IgG Fc receptors.Fluorescence of this long-wavelength Alexa Fluor dye is not visible by looking through a conventional fluorescence microscope.
Product will be shipped at Room Temperature.
靶标信息
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
