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Cholesterol Oxidase Antibody (200-101-213-0100) in WB
Rockland Goat anti Cholesterol oxidase antibody (200-101-213 lot 6534) was used to detect purified cholesterol oxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified cholesterol oxidase contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with a 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/M... View More
产品信息
200-101-213-0100
应用
建议稀释比
已发表文章
产品规格
种属反应
Bacteria
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Cholesterol Oxidase [Streptomyces]
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Ion-exchange chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-CHOLESTEROL OXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Cholesterol Oxidase [Microorganism]. Cross reactivity against Cholesterol Oxidase from other sources is unknown.
靶标信息
Cholesterol Oxidase is a bifunctional enzyme that catalyzes the oxidation of the 3-beta-hydroxy group of cholesterol and the isomerization of the double bond of the resulting product. This protein is involved in the pathway cholesterol metabolism, which is part of Steroid metabolism.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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