Invitrogen

Claudin 1 Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1个已发表图片

1 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

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Claudin 1 Antibody (PA1-37465) in WB

Western blot was performed using Anti-CLDN1 Polyclonal Antibody (Product # PA1-37465) and a 22 kDa band corresponding to CLDN1 was observed across cell lines tested and increased upon EGF, LiCl treatment and IL-beta treatment. Membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1), MDCK (Lane 2), A-431 (Lane 3), HEK-293 (Lane 4), Caco-2 treated with EGF (100 ng/mL) and LiCl (50mM) simultaneously for 24 Hours (Lane 5), Caco-2 treated with IL-beta (10 ng/mL for 48 Hours) (Lane 6), Caco-2 (Lane 7), tissue extracts (30 µg lysate) of Mous... View More

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产品信息

PA1-37465

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化（石蜡） (IHC (P))

1:200

免疫组化（冰冻） (IHC (F))

1:200

免疫细胞化学 (ICC/IF)

5 µg/mL

产品规格

种属反应

Dog, Human, Mouse

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from C-terminus of human claudin-1 peptide

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. not determined

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.6, with 1% BSA

内含物

0.1% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2244877

产品详细信息

This antibody is predicted to react with bovine, canine, porcine and rat based on sequence homology.

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is skin, breast carcinoma or ovarian carcinoma.

靶标信息

Claudin-1 is a member of the transmembrane protein family claudins located in cell-cell tight junctions and it acts as a co-receptor for HCV entry into hepatic cells. Claudins are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Claudin-1 is expressed in most tissues such as bladder, fallopian tube, liver, pancreas, prostate, and skin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。