Invitrogen

Collagen IV Polyclonal Antibody

Collagen IV Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Collagen IV Antibody (PA5-95188) in IHC (P)

Immunohistochemistry analysis of Collagen IV in paraffin-embedded human lung cancer tissues. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with Collagen IV polyclonal antibody (Product # PA5-95188) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biot... View More

Collagen IV Antibody in Western Blot (WB)

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产品信息

PA5-95188

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human Collagen IV recombinant protein (Position: G1445-T1669).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2806993

产品详细信息

Human Collagen IV shares 97% amino acid (aa) sequence identity with mouse Collagen IV.

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

Collagen IV is a type of collagen protein that is a major component of the basement membrane in various tissues, including the kidneys, lungs, and skin. It forms a network-like structure that provides support and stability to the surrounding tissue. Collagen IV is composed of three alpha chains, which are encoded by the COL4A1, COL4A2, and COL4A3 genes. Mutations in these genes can lead to various genetic disorders, such as Alport syndrome and Goodpasture syndrome, which affect the kidneys and lungs, respectively.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: arresten; COL4A1 NC1 domain; Collagen 4; Collagen alpha-1(IV) chain; Collagen alpha-2(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; Collagen alpha-5(IV) chain; Collagen alpha-6(IV) chain; collagen IV, alpha-1 polypeptide; collagen IV, alpha-3 polypeptide; Collagen IV, alpha-4 polypeptide; collagen IV, alpha-5 polypeptide; collagen IV, alpha-6 polypeptide; collagen of basement membrane, alpha-1 chain; collagen of basement membrane, alpha-4 chain; collagen of basement membrane, alpha-5 chain; collagen of basement membrane, alpha-6; collagen, type IV, alpha 1; collagen, type IV, alpha 3 (Goodpasture antigen); collagen, type IV, alpha 4; collagen, type IV, alpha 5; collagen, type IV, alpha 6; dA149D17.3; dA24A23.1; dJ889N15.4 (Collagen Alpha 6(IV)); Goodpasture antigen; tumstatin; Type IV Collagen

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基因别名: ASLN; ATS; BSVD; CA44; CA54; COL4A1; COL4A2; COL4A3; COL4A4; COL4A5; COL4A6; CXDELq22.3; DELXq22.3; DFNX6; ICH; POREN2; RATOR