Invitrogen

Crk Polyclonal Antibody

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抗体检测数据 (2)

Crk Antibody in Immunocytochemistry (ICC/IF)

Crk Antibody in Immunocytochemistry (ICC/IF)

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Crk Antibody (PA5-112391) in ICC/IF

Immunocytochemichal analysis of Crk in A549 cells using a Polyclonal antibody (Product # PA5-112391) at a dilution of 1:100. An Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibdoy was used.

Crk Antibody in Immunocytochemistry (ICC/IF)

Crk Antibody in Western Blot (WB)

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Crk Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemichal analysis of Crk in A549 cells using a Polyclonal antibody (Product # PA5-112391) at a dilution of 1:100. An Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibdoy was used.

Crk Antibody in Western Blot (WB) — Western Blot analysis of Crk in multiple sample types using a Crk polyclonal antibody (Product # PA5-112391) at a concentration of 3 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000. Sample types that were tested include: HepG2 whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate, Hela whole cell lysate, HEK293 whole cell lysate .

产品信息

PA5-112391

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:5,000

-

免疫细胞化学 (ICC/IF)

1:50-1:200

-

酶联免疫吸附实验 (ELISA)

Assay-dependent

-

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Human Adapter molecule crk protein (40-196AA)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

3 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.03% ProClin 300

保存条件

-20°C or -80°C if preferred

运输条件

Wet ice

RRID

AB_2867126

靶标信息

The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk- II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Adapter molecule crk; avian sarcoma virus CT10 (v-crk) oncogene homolog; CSAID-binding protein; Csaids binding protein; cytokine suppressive anti-inflammatory drug binding protein; Cytokine suppressive anti-inflammatory drug-binding protein; cytokine-supressive anti-inflammatory drug binding protein; MAP kinase 14; MAP kinase 2; MAP kinase MXI2; MAP kinase p38 alpha; MAPK 14; MAX-interacting protein 2; Mitogen-activated protein kinase 14; mitogen-activated protein kinase 14A; Mitogen-activated protein kinase p38 alpha; p38; p38 MAP kinase; p38 mitogen activated protein kinase; p38alpha Exip; Proto-oncogene c-Crk; reactive kinase; SAPK2a; Stress-activated protein kinase 2a; v-crk avian sarcoma virus CT10 oncogene homolog; v-crk sarcoma virus CT10 oncogene homolog; v-crk sarcoma virus CT10 oncogene-like protein

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基因别名: CRK; CRK1; CRKII; Crko; CSBP; CSBP1; CSBP2; CSPB1; EXIP; Hog; MAPK14; MXI2; p38; p38ALPHA; p38Hog; PRKM14; PRKM15; RK; SAPK2A

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UniProt ID: (Human) P46108, (Rat) Q63768, (Human) Q16539

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Entrez Gene ID: (Human) 1398, (Rat) 54245, (Human) 1432, (Rat) 81649

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SH3/SH2 adaptor activity protein binding SH2 domain binding protein phosphorylated amino acid binding ephrin receptor binding protein domain specific binding protein binding, bridging protein serine/threonine kinase activity MAP kinase activity MAP kinase kinase activity ATP binding protein C-terminus binding enzyme binding protein phosphatase binding NFAT protein binding protein kinase activity kinase activity scaffold/adaptor protein non-receptor serine/threonine protein kinase

参与通路

activation of MAPKK activity regulation of transcription from RNA polymerase II promoter regulation of actin cytoskeleton organization regulation of Rac protein signal transduction Fc-gamma receptor signaling pathway involved in phagocytosis regulation of GTPase activity vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway positive regulation of substrate adhesion-dependent cell spreading activation of JUN kinase activity positive regulation of signal transduction regulation of cellular component movement DNA damage checkpoint activation of MAPK activity cell morphogenesis cartilage condensation angiogenesis placenta development chondrocyte differentiation glucose metabolic process transcription, DNA-templated apoptotic process movement of cell or subcellular component chemotaxis signal transduction cell surface receptor signaling pathway transmembrane receptor protein serine/threonine kinase signaling pathway Ras protein signal transduction skeletal muscle tissue development response to glucose positive regulation of gene expression positive regulation of myotube differentiation myoblast differentiation involved in skeletal muscle regeneration peptidyl-serine phosphorylation fatty acid oxidation osteoclast differentiation positive regulation of cyclase activity lipopolysaccharide-mediated signaling pathway response to muramyl dipeptide intracellular signal transduction cellular response to vascular endothelial growth factor stimulus response to muscle stretch p38MAPK cascade positive regulation of protein import into nucleus signal transduction in response to DNA damage positive regulation of blood vessel endothelial cell migration positive regulation of erythrocyte differentiation positive regulation of myoblast differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import protein autophosphorylation regulation of sequence-specific DNA binding transcription factor activity striated muscle cell differentiation positive regulation of muscle cell differentiation positive regulation of cardiac muscle cell proliferation 3'-UTR-mediated mRNA stabilization cellular response to lipopolysaccharide cellular response to ionizing radiation negative regulation of canonical Wnt signaling pathway positive regulation of brown fat cell differentiation stress-induced premature senescence cellular response to virus regulation of cytokine production involved in inflammatory response positive regulation of myoblast fusion regulation of signal transduction by p53 class mediator positive regulation of reactive oxygen species metabolic process positive regulation of interleukin-12 secretion MAPK cascade regulation of transcription, DNA-templated protein phosphorylation cellular response to DNA damage stimulus response to lipopolysaccharide stress-activated MAPK cascade