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Cyclin B1 Antibody (500-4104) in IHC (P)
Immunohistochemistry: Human Colon carcinoma (FFPE) stained with Rabbit anti-Cyclin B1 antibody (Cat# 500-4104) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
产品信息
500-4104
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide derived from C-terminus of human cyclin B1 protein
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with proprietary stabilizer
内含物
0.01% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Positive control: Carcinoma
Cellular location: Cytoplasm, Nucleus.
靶标信息
Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory protein involved in mitosis. It is essential for the control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. Destruction of Cyclin B1 is required for cell cycle progression. Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: cyclin B1, related sequence 1; cyclin B1, related sequence 13; G2/mitotic-specific cyclin B1; G2/mitotic-specific cyclin-B1; OTTHUMP00000221981; OTTHUMP00000223023
基因别名: Ccn-2; CCNB; CCNB1; Ccnb1-rs1; Ccnb1-rs13; Cycb; Cycb-4; Cycb-5; CycB1; Cycb1-rs1
UniProt ID: (Human) P14635, (Rat) P30277, (Mouse) P24860
Entrez Gene ID: (Human) 891, (Rat) 25203, (Mouse) 268697
G2/M transition of mitotic cell cycle
oocyte maturation
in utero embryonic development
negative regulation of protein phosphorylation
protein complex assembly
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
mitotic spindle organization
mitotic nuclear envelope disassembly
mitotic metaphase plate congression
spermatogenesis
response to mechanical stimulus
negative regulation of gene expression
anaphase-promoting complex-dependent catabolic process
positive regulation of mRNA 3'-end processing
positive regulation of histone phosphorylation
tissue regeneration
response to drug
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
positive regulation of mitotic cell cycle
response to DDT
positive regulation of fibroblast proliferation
digestive tract development
cell division
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
regulation of cell cycle
positive regulation of attachment of spindle microtubules to kinetochore
ventricular cardiac muscle cell development
positive regulation of cardiac muscle cell proliferation
regulation of chromosome condensation
cellular response to iron(III) ion
cellular response to fatty acid
cellular response to organic cyclic compound
cellular response to hypoxia
regulation of mitotic cell cycle spindle assembly checkpoint
histone H3-S10 phosphorylation involved in chromosome condensation
protein phosphorylation
cell cycle
mitotic nuclear division
positive regulation of cell cycle
