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产品信息
MA5-15650
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
3D8
抗原
Purified recombinant fragment of human CCND1 expressed in E. Coli.
偶联物
形式
Liquid
浓度
Conc. Not Determined
保存液
ascites
内含物
0.03% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_10978307
产品详细信息
MA5-15650 targets CCND1 in indirect ELISA, WB applications and shows reactivity with Human samples.
The MA5-15650 immunogen is purified recombinant fragment of human CCND1 expressed in E. Coli.
.
MA5-15650 detects CCND1 which has a predicted molecular weight of approximately 33.7kDa.
靶标信息
Cyclin D1 (PRAD-1, bcl-1) is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein. Cyclin D1 is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL). In addition, cyclin D1 positively regulates protein phosphorylation, mammary gland epithelial cell proliferation, and fat cell differentiation. In humans, the CCND1 gene encoding cyclin D1 is present on chromosome 11. Cyclin D1 is a cytoplasmic and nuclear protein that is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Without growth factor-mediated stimulation, Cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest. The Cyclin D1 protein belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The Cyclin D1 protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Cyclin D1 has been successfully employed and is a promising tool for further studies in both cell cycle biology and cancer associated abnormalities.
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生物信息学
蛋白别名: B-cell CLL/lymphoma 1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; CCND1 protein; cDNA; FLJ93625; G1/S-specific cyclin-D1; mRNA; PRAD-1; PRAD1 oncogene
基因别名: BCL1; CCND1; D11S287E; PRAD1; U21B31
UniProt ID: (Human) P24385
Entrez Gene ID: (Human) 595
transcription corepressor activity
protein kinase activity
protein binding
transcription factor binding
cyclin-dependent protein serine/threonine kinase regulator activity
enzyme binding
protein kinase binding
protein complex binding
histone deacetylase binding
proline-rich region binding
kinase activator
G1/S transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
re-entry into mitotic cell cycle
positive regulation of protein phosphorylation
transcription, DNA-templated
protein phosphorylation
cellular response to DNA damage stimulus
lactation
response to iron ion
response to X-ray
response to organonitrogen compound
positive regulation of G2/M transition of mitotic cell cycle
negative regulation of Wnt signaling pathway
negative regulation of epithelial cell differentiation
endoplasmic reticulum unfolded protein response
mitotic G1 DNA damage checkpoint
response to magnesium ion
response to estradiol
response to vitamin E
Leydig cell differentiation
mammary gland epithelial cell proliferation
positive regulation of mammary gland epithelial cell proliferation
response to drug
response to estrogen
response to leptin
fat cell differentiation
response to ethanol
positive regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of cell cycle
cell division
response to corticosterone
response to calcium ion
canonical Wnt signaling pathway
mammary gland alveolus development
response to UV-A
negative regulation of cell cycle arrest
liver regeneration
