NeoBiotechnologies

Cyclin E (G1/S-Phase Cyclin) Monoclonal Antibody (CCNE1/2460)

Cyclin E (G1/S-Phase Cyclin) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Cyclin E (G1/S-Phase Cyclin) Antibody (898-MSM1-P1ABX) in IHC (P)

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cyclin E Mouse Monoclonal Antibody (CCNE1/2460).

Cyclin E (G1/S-Phase Cyclin) Antibody in Western Blot (WB)

Cyclin E (G1/S-Phase Cyclin) Antibody in Peptide array (ARRAY)

Cyclin E (G1/S-Phase Cyclin) Antibody in SDS-PAGE (SDS-PAGE)

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产品信息

898-MSM1-P1ABX

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

免疫组化（石蜡） (IHC (P))

Assay-dependent

免疫组织化学（多聚甲醛固定） (IHC (PFA))

1-2 µg/mL

免疫细胞化学 (ICC/IF)

1-2 µg/mL

流式细胞分析 (Flow)

1-2 µg/10^6 cells

肽阵列 (Array)

Assay-dependent

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

CCNE1/2460

抗原

Recombinant human Cyclin E (CCNE1) protein fragment (around aa 10-176).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A/G

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

靶标信息

Cyclin E belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.

仅用于科研。不用于诊断过程。未经明确授权不得转售。