NeoBiotechnologies

Cyclin E (G1/S-Phase Cyclin) Recombinant Mouse Monoclonal Antibody (rCCNE1, 4936)

Cyclin E (G1/S-Phase Cyclin) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Cyclin E (G1/S-Phase Cyclin) Antibody (898-MSM6-P1ABX) in IHC (P)

Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human ovarian carcinoma using Cyclin E (G1, S-Phase Cyclin) Recombinant Monoclonal Antibody (Product # 898-MSM6-P1ABX).

Cyclin E (G1/S-Phase Cyclin) Antibody in Western Blot (WB)

Cyclin E (G1/S-Phase Cyclin) Antibody in SDS-PAGE (SDS-PAGE)

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产品信息

898-MSM6-P1ABX

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

免疫组化（石蜡） (IHC (P))

1-2 µg/mL

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

rCCNE1, 4936

抗原

Recombinant human Cyclin E (CCNE1) protein fragment (around aa 10-176)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A/G

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20°C or -80°C if preferred

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

靶标信息

Cyclin E belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.

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