Invitrogen

Cyp4a10 Polyclonal Antibody

Cyp4a10 Antibody in Immunocytochemistry (ICC/IF)

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Cyp4a10 Antibody (PA3-033) in ICC/IF

Immunofluorescent analysis of Cytochrome P450 4A (green) showing staining in the cytoplasm and membrane of H-4-II-E cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 4A polyclonal antibody (Product # PA3-033) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS... View More

Cyp4a10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA3-033

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200-1:2,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:500

免疫组化（冰冻） (IHC (F))

1:100

免疫细胞化学 (ICC/IF)

1:20-1:200

免疫沉淀 (IP)

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产品规格

种属反应

Cat, Human, Rabbit, Rat

已发表种属

Hamster, Mouse, Pig, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues D(431) P S R F A P D S P R H S H S(445) of rat Cytochrome P450 4A.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

whole serum, PBS

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_325980

产品详细信息

PA3-033 detects cytochrome P450 4A (isoforms 1, 2, & 3) from human, feline, rabbit and rat tissues.

PA3-033 has been successfully used in Western blot, ICC/IF and IHC procedures. By Western blot, this antibody detects an ~50 kDa protein representing P450 4A in rat liver extract. Immunohistochemical staining of P450 4A in rat brain with PA3-033 results in perivascular and surrounding cortex staining.

The PA3-033 immunogen is a synthetic peptide corresponding to residues D(431) P S R F A P D S P R H S H S(445) of rat Cytochrome P450 4A.

靶标信息

The cytochrome P450 (P450) family of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as NADPH and P450 reductase. There are over 200 cDNA’s which encode P450 protein. Epoxygenases are those P450 proteins which metabolize AA to epoxyeicosatrienoic acid (EETs) and w-hydroxylases are those P450 proteins which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). 20-HETE is converted from AA by the 4A family of P450 proteins which includes at least 8 different, though closely related isoforms. 4A1, 4A2, & 4A3 have been cloned from liver, kidney and testis and have been detected in renal, hepatic & brain microvessels.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Proteomics coupled transcriptomics reveals lipopolysaccharide inhibiting peroxisome proliferator-activated receptors signalling pathway to reduce lipid droplets accumulation in mouse liver.

Proteomics

Ran H,Sun W,Wang L,Wang X,Yu H,Chen J,Liu F,Chao Z,Pu Q,Liu Y,Zeng Y,Li Z,Wan Y,Yuan J

PA3-033 was used in Western Blot to find that lipopolysaccharide (LPS) stimulation reduced the LDs content in the mouse liver.

Sun Oct 01 00:00:00 EDT 2023

Acquired deficiency of peroxisomal dicarboxylic acid catabolism is a metabolic vulnerability in hepatoblastoma.

The Journal of biological chemistry

Wang H,Lu J,Chen X,Schwalbe M,Gorka JE,Mandel JA,Wang J,Goetzman ES,Ranganathan S,Dobrowolski SF,Prochownik EV

Published figure using Cyp4a10 polyclonal antibody (Product # PA3-033) in Western Blot

Wed Aug 25 00:00:00 EDT 2021

The peroxisomal transporter ABCD3 plays a major role in hepatic dicarboxylic fatty acid metabolism and lipid homeostasis.

Journal of inherited metabolic disease

Ranea-Robles P,Chen H,Stauffer B,Yu C,Bhattacharya D,Friedman SL,Puchowicz M,Houten SM

PA3-033 was used in Western Blot to provide data on the role of the peroxisomal transporter ABCD3 in hepatic lipid homeostasis and DCA metabolism, and the consequences of peroxisomal dysfunction for the liver.

Mon Nov 01 00:00:00 EDT 2021

Expression of CYP 4A ω-hydroxylase and formation of 20-hydroxyeicosatetreanoic acid (20-HETE) in cultured rat brain astrocytes.

Prostaglandins & other lipid mediators

Gebremedhin D,Zhang DX,Carver KA,Rau N,Rarick KR,Roman RJ,Harder DR

PA3-033 was used in immunocytochemistry and western blot to utilize cultured rat brain astrocytes to study the expression of CYP 4A omega-hydroxylase and formation of 20-hydroxyeicosatetreanoic acid (20-HETE)

Fri Jul 01 00:00:00 EDT 2016

Beneficial effects of β-conglycinin on renal function and nephrin expression in early streptozotocin-induced diabetic nephropathy rats.

The British journal of nutrition

Yang HY,Wu LY,Yeh WJ,Chen JR

PA3-033 was used in western blot to study the effects of beta-conglycin and soya isoflavone compounds in a rat model of diabetic nephropathy

Tue Jan 14 00:00:00 EST 2014

Effects of soy protein on alcoholic liver disease in rats undergoing ethanol withdrawal.

The Journal of nutritional biochemistry

Yang HY,Lin HS,Chao JC,Chien YW,Peng HC,Chen JR

PA3-033 was used in western blot to investigate the effectiveness of soy protein in decreasing the level of ethanol on alcoholic liver disease

Fri Jun 01 00:00:00 EDT 2012

Attenuation of neonatal ischemic brain damage using a 20-HETE synthesis inhibitor.

Journal of neurochemistry

Yang ZJ,Carter EL,Kibler KK,Kwansa H,Crafa DA,Martin LJ,Roman RJ,Harder DR,Koehler RC

PA3-033 was used in immunohistochemistry to investigate the effect of 20-hydroxyeicosatetraenoic acid synthesis inhibitor HET0016 on neonatal ischemic brain damage

Sun Apr 01 00:00:00 EDT 2012

Modulation by salt intake of the vascular response mediated through adenosine A(2A) receptor: role of CYP epoxygenase and soluble epoxide hydrolase.

American journal of physiology. Regulatory, integrative and comparative physiology

Nayeem MA,Zeldin DC,Boegehold MA,Morisseau C,Marowsky A,Ponnoth DS,Roush KP,Falck JR

PA3-033 was used in western blot to investigate the role of adenosine A(2A) receptor in the vascular response modulated by high salt intake

Thu Jul 01 00:00:00 EDT 2010

7-Benzyloxyresorufin-O-dealkylase activity as a marker for measuring cytochrome P450 CYP3A induction in mouse liver.

Analytical biochemistry

Hagemeyer CE,Bürck C,Schwab R,Knoth R,Meyer RP

PA3-033 was used in western blot to investigate the relationship between 7-Benzyloxyresorufin-O-dealkylase activity and cytochrome P450 CYP3A induction in mouse liver

Mon Mar 01 00:00:00 EST 2010

Small G proteins in peroxisome biogenesis: the potential involvement of ADP-ribosylation factor 6.

BMC cell biology

Anthonio EA,Brees C,Baumgart-Vogt E,Hongu T,Huybrechts SJ,Van Dijck P,Mannaerts GP,Kanaho Y,Van Veldhoven PP,Fransen M

PA3-033 was used in western blot to investigate the role of ADP-ribosylation factor 6 (Arf6) in peroxisome biogenesis

Mon Aug 17 00:00:00 EDT 2009

High dietary salt reduces the contribution of 20-HETE to arteriolar oxygen responsiveness in skeletal muscle.

American journal of physiology. Heart and circulatory physiology

Marvar PJ,Falck JR,Boegehold MA

PA3-033 was used in western blot to investigate the effects of high dietary salt on arteriolar oxygen responsiveness in skeletal muscle

Thu Mar 01 00:00:00 EST 2007

Potential role for Pex19p in assembly of PTS-receptor docking complexes.

The Journal of biological chemistry

Fransen M,Vastiau I,Brees C,Brys V,Mannaerts GP,Van Veldhoven PP

PA3-033 was used in immunoprecipitation to investigate the role of Pex19p during the assembly of PTS-receptor docking complexes.

Fri Mar 26 00:00:00 EST 2004

Altered renal P-450 metabolism of arachidonic acid in Dahl salt-sensitive rats.

The American journal of physiology

Ma YH,Schwartzman ML,Roman RJ

PA3-033 was used in western blot to investigate the metabolism of arachidonic acid by P-450 in Dahl salt-sensitive and salt-resistant rats

Mon Aug 01 00:00:00 EDT 1994

生物信息学

蛋白别名: CYP; CYPIVA10; Cytochrome P450 4A10; cytochrome P450, 4A1; cytochrome P450, 4a10; cytochrome P450, family 4, subfamily a, polypeptide 10; cytochrome P450, family 4, subfamily A, polypeptide 22; cytochrome P450, IVA1; Cytochrome P450-LA-omega 1; Cytochrome P452; Lauric acid omega-hydroxylase; Long-chain fatty acid omega-monooxygenase; P450

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基因别名: Cyp4a-1; Cyp4a1; Cyp4a10; Cyp4a22; CYPIVA10

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UniProt ID: (Rat) P08516

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Entrez Gene ID: (Rat) 50549

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功能

monooxygenase activity iron ion binding arachidonic acid monooxygenase activity arachidonic acid epoxygenase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen alkane 1-monooxygenase activity heme binding leukotriene-B4 20-monooxygenase activity arachidonic acid omega-hydroxylase activity oxygenase

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