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Cypridina luciferase Antibody (PA1-182) in WB
Western blot analysis of secreted Cypridina luciferase was performed using 293T cells stably expressing Gaussia or Cypridina luciferase. Cells were grown overnight in serum free (B27 supplemented) DMEM with or without 10uM Brefeldin A. Equal amounts (75 µg) of approximately 10x concentrated cell culture medium from treated or untreated cells, and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616... View More
产品信息
PA1-182
产品规格
种属反应
Luciferase
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Purified recombinant Cypridina luciferase expressed in E. coli.
偶联物
Unconjugated
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS with 1mg/mL BSA, 30% glycerol
内含物
0.05% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2539913
产品详细信息
Western blot analysis of PA1-182 detects a ~62 kDa protein in the supernatant of Cypridina luciferase-secreting cells.
PA1-182 can be used for activity-independent verification of Cypridina luciferase expression.
靶标信息
The Cypridina luciferase is a 62kDa protein from the marine ostracod, Cypridina noctiluca. The bioluminescent enzyme is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. Light output generated by the luciferase reaction can be correlated to the amount of Cypridina luciferase protein produced and used to determine the activity of the promoter driving Cypridina expression. Cypridina luciferase can be measured individually or in multiplex assays, such as the Cypridina-Firefly Luciferase Dual Assay Kit (Product # 16183). The Cypridina luciferase has been codon optimized for enhanced performance.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Cypridina noctiluca luciferase
