Invitrogen

Cytochrome C Polyclonal Antibody

Cytochrome C Antibody in Immunocytochemistry (ICC/IF)

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Cytochrome C Antibody (PA5-79120) in ICC/IF

Immunocytochemistry analysis of CYCS using anti-CYCS antibody (Product # PA5-79120) . CYCS was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5μg/mL rabbit anti-CYCS antibody (Product # PA5-79120) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG... View More

Cytochrome C Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cytochrome C Antibody in Immunohistochemistry (Frozen) (IHC (F))

Cytochrome C Antibody in Western Blot (WB)

Cytochrome C Antibody in Flow Cytometry (Flow)

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产品信息

PA5-79120

应用

建议稀释比

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免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫组化（冰冻） (IHC (F))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

1- µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence at the C-terminus of human Cytochrome C (91-105 aa ERADLIAYLKKATNE).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide, 0.05mg thimerosal

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2746236

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。