Invitrogen

Cytochrome C Monoclonal Antibody (6H2.B4), Alexa Fluor™ 488

Cytochrome C Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 1

Cytochrome C Antibody (MA3-38200-A488) in ICC/IF

Immunofluorescent analysis of Cytochrome C (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Cytochrome C Monoclonal Antibody, AlexaFluor 488 conjugate (Product # MA7-00184-A488) at a dilution of 5 µg/mL in blocking buffer for 1 hour at ... View More

Product Image Gallery

产品信息

MA3-38200-A488

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

1-10 µg/mL

流式细胞分析 (Flow)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Fruit fly, Human, Mouse, Rabbit, Rat

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

6H2.B4

抗原

Rat cytochrome C

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2663051

产品详细信息

This antibody reacts with native Cytochrome C, but does not recognize denatured cytochrome C. The antibody recognizes a region in the vicinity of amino acid 62 of rat Cytochrome C.

靶标信息

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A new quantitative assay for cytochrome c release in apoptotic cells.

Cell death and differentiation

Waterhouse NJ,Trapani JA

MA3-38200-A488 was used in Flow Cytometry to help highlight some of the problems associated with current methods to follow cytochrome c release.

Tue Jul 01 00:00:00 EDT 2003

生物信息学

蛋白别名: CG17903-PA; CG17903-PB; Cyt-c-p-PA; Cyt-c-p-PB; Cytochrome c; cytochrome c DC4; Cytochrome C, expressed in somatic tissues; Cytochrome c, somatic; Cytochrome c-2; Cytochrome c-proximal; cytochrome-c-p; Cytochrome-c-proximal; hc1; lethal (2) k13905; RH17228p

查看更多收起

基因别名: CG17903; CYC; CYC2; CYCS; CYCSA; Cyct-c-p; Cyt C; cyt c-p; Cyt-c; Cyt-c-p; Cyt-c2; cyt.c; CytC; CytC-2; Cytc-p; CYTC2; cytochrome c; DC4; dCyt.C-p; DMc01; Dmel\CG17903; Dmel_CG17903; HCS; l(2)k13905; pDMc01; THC4