Invitrogen

Cytokeratin 14 Monoclonal Antibody (A2C10)

Cytokeratin 14 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Cytokeratin 14 Antibody (MA5-42399) in IHC (P)

Immunohistochemistry (Paraffin) analysis of paraffin-embedded human tonsil tissue using Cytokeratin 14 Monoclonal Antibody (Product # MA5-42399). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the Cytokeratin 14 antibody ... View More

Cytokeratin 14 Antibody in Western Blot (WB)

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产品信息

MA5-42399

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:200-1:1000

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

A2C10

抗原

Synthetic peptide within Human Cytokeratin 14 aa 423-472/472

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 50% glycerol, 0.2% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2911540

产品详细信息

Positive Control: A431 cell lysates, human tonsil tissue, human skin tissue.

Subcellular Location: Cytoplasm, Nucleus.

靶标信息

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie. skin) and non-keratinizing cells (ie. mesothelial cells). At least 20 different CKs can be identified. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). Belonging to the type I subfamily of low molecular weight keratins and existing in combination with keratin 5, keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and is useful in identification of squamous cell carcinomas. It is considered a prognostic marker in breast carcinomas. At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。