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Cytokeratin 19 Antibody (MA1-19478) in WB
Western blotting analysis of human cytokeratin 19 using mouse monoclonal antibody BA-17 (Product # MA1-19478) on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of biotinylated mouse anti-cytokeratin 19 monoclonal antibody followed by IRDye800-conjugated streptavidin. Cytokeratin 19 was detected at approximately 41kDa.
产品信息
MA1-19478
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human,
Rat
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
BA-17
抗原
Mammary organoids.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Size-exclusion chromatography
保存液
PBS, pH 7.4
内含物
15mM sodium azide
保存条件
4° C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1075172
产品详细信息
This antibody reacts with cytokeratin 19 (40 kDa intracellular antigen) in human tissue. Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with cytokeratin 7.
靶标信息
Cytokeratin 19 is part of a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight from 40-68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. For example, cytokeratin 19 is not expressed in hepatocytes, therefore, it is useful in the identification of liver metastasis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 40-kDa keratin intermediate filament; C K1CS; CK 19; CK-19; cytokeratin 19; Cytokeratin-19; K19; keratin 19, type I; Keratin, type I cytoskeletal 19; keratin, type I, 40-kd; Keratin-19; MGC15366
基因别名: CK19; K19; K1CS; KRT19
UniProt ID: (Human) P08727
Entrez Gene ID: (Human) 3880
