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DACT1 Antibody (CF809156) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DACT1 (RC212564, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-DACT1. (1:2000)
产品信息
CF809156
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
OTI13H4
抗原
Human recombinant protein fragment corresponding to amino acids 289-555 of human DACT1 produced in E.coli.
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3, with 8% trehalose
内含物
no preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
靶标信息
Dapper homolog 1, an ortholog of Xenopus Dpr, antagonizes Wnt signaling by promoting Dvl degradation via a lysosome inhibitor-sensitive and proteasome inhibitor-insensitive mechanism. Dapper homolog 1 acts as an interacting protein for Dvl, and the interaction is formed between the DEP (Dishevelled, Egl-10, and pleckstrin) domain of Dvl and the central and C-terminal regions of Dapper homolog 1. The gene for human Dapper homolog 1 is localized on chromosomal region 14q23.1. The protein impedes the degradation of CTNNB1/beta-catenin, thereby enhancing the transcriptional activation of target genes of the Wnt signaling pathway. The protein is also required for normal notochord formation and is downregulated in hepatocellular carcinomas (HCC). It is expressed in human liver and various other tissues.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Dapper antagonist of catenin 1; Dapper homolog 1; dapper, antagonist of beta-catenin, homolog 1; dishevelled-binding antagonist of beta-catenin 1; hDPR1; Hepatocellular carcinoma novel gene 3 protein; heptacellular carcinoma novel gene 3
基因别名: DACT1; DAPPER; DAPPER1; DPR1; FRODO; HDPR1; HNG3; THYEX3
UniProt ID: (Human) Q9NYF0
Entrez Gene ID: (Human) 51339
negative regulation of transcription from RNA polymerase II promoter
Wnt signaling pathway
neural tube development
positive regulation of Wnt signaling pathway
negative regulation of Wnt signaling pathway
regulation of protein stability
negative regulation of protein binding
positive regulation of protein binding
regulation of catenin import into nucleus
positive regulation of catenin import into nucleus
positive regulation of protein catabolic process
negative regulation of JNK cascade
embryonic hindgut morphogenesis
negative regulation of canonical Wnt signaling pathway
regulation of nodal signaling pathway
positive regulation of cellular protein catabolic process
negative regulation of beta-catenin-TCF complex assembly
regulation of Wnt signaling pathway, planar cell polarity pathway
negative regulation of G1/S transition of mitotic cell cycle
