Invitrogen

DCK Polyclonal Antibody

DCK Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 9

DCK Antibody (PA5-79140) in ICC/IF

Immunocytochemistry analysis of DCK using anti-DCK antibody (Product # PA5-79140) . DCK was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-DCK antibody (Product # PA5-79140) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filte... View More

DCK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DCK Antibody in Immunohistochemistry (Frozen) (IHC (F))

Product Image Gallery

产品信息

PA5-79140

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫组化（冰冻） (IHC (F))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

0.5-1 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived human DCK recombinant protein (Position: E17-L260).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2746256

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。