Invitrogen

DENR Polyclonal Antibody

DENR Antibody in Immunocytochemistry (ICC/IF)

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DENR Antibody (PA5-112241) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of DENR in PC3 cells. Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with DENR Polyclonal Antibody (Product # PA5-112241) (1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm and cell membrane.

DENR Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DENR Antibody in Immunoprecipitation (IP)

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产品信息

PA5-112241

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

免疫沉淀 (IP)

0.5-2 µL/mg of lysate

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived Human DENR fragment

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography, Protein A

保存液

PBS, pH 7

内含物

0.03% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2866977

靶标信息

This gene encodes a protein whose expression was found to increase in cultured cells at high density but not during growth arrest. This gene was also shown to have increased expression in cells overexpressing HER-2/neu proto-oncogene. The protein contains an SUI1 domain. In budding yeast, SUI1 is a translation initiation factor that along with eIF-2 and the initiator tRNA-Met, directs the ribosome to the proper translation start site. Proteins similar to SUI have been found in mammals, insects, and plants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。