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DGKA Antibody (PA5-89555) in WB
Western blot analysis of DGKA in extracts of various cell lines. Samples were incubated with DGKA Polyclonal antibody (Product # PA5-89555) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
产品信息
PA5-89555
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human DGKA (NP_0013362)
偶联物
Unconjugated
形式
Liquid
浓度
0.55 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.09% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2805648
产品详细信息
Immunogen sequence: MAKERGLISP SDFAQLQKYM EYSTKKVSDV LKLFEDGEMA KYVQGDAIGY EGFQQFLKIY LEVDNVPRHL SLALFQSFET GHCLNETNVT KDVVCLNDVS CYFSLLEGGR PEDKLEFTFK LYDTDRNGIL DSSEVDKIIL QMMRVAEYLD WDVSELRPIL QEMMKEIDYD GSGSVSQAEW VRAGATTVPL LVLLGLEMTL; Positive Samples: NCI-H460, MCF-7, HeLa, Mouse lung, Rat lung
靶标信息
The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 80 kDa diacylglycerol kinase; DAG kinase alpha; DGK-alpha; Diacylglycerol kinase alpha; diacylglycerol kinase alpha Flt2-Pr2; diacylglycerol kinase, alpha 80kDa; Diglyceride kinase alpha; MGC12821; MGC42356; truncated diacylglycerol kinase alpha Flt1-Pr1; truncated diacylglycerol kinase alpha Flt1-Pr2
基因别名: 80kDa; AW146112; DAGK; DAGK1; DGK-alpha; DGKA
UniProt ID: (Human) P23743, (Mouse) O88673, (Rat) P51556
Entrez Gene ID: (Human) 1606, (Mouse) 13139, (Rat) 140866
