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DIO1 Antibody (PA5-100139) in ICC/IF
Immunofluorescent analysis of DIO1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and DIO1 polyclonal antibody (Product # PA5-100139) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
产品信息
PA5-100139
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human DIO1(Accession P49895), corresponding to amino acid residues Q220-S249.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2815669
产品详细信息
Antibody detects endogenous levels of total DIO1.
靶标信息
The protein encoded by this gene is a thiol-requiring propylthiouracil-sensitive oxidoreductase. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). It also degrades both hormones by inner ring deiodination (IRD). Alternative splicing results in multiple transcript variants encoding different isoforms. Some, but not all, isoforms contain a selenocysteine (Sec) residue encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Additional transcript variants have been described but are not supported by experimental evidence.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 5DI; DIOI; hDido1; iodothyronine deiodinase type 1; thyroxine 5'-deiodinase; thyroxine deiodinase; thyroxine deiodinase type I (selenoprotein); thyroxine deiodinase, type I; Type 1 DI; Type I iodothyronine deiodinase; Type-I 5'-deiodinase
基因别名: 5DI; D1; DIO1; ITDI1; TXDI1
UniProt ID: (Human) P49895, (Mouse) Q61153, (Rat) P24389
Entrez Gene ID: (Human) 1733, (Mouse) 13370, (Rat) 25430
