Invitrogen

DISC1 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

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3 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

DISC1 Antibody in Immunocytochemistry (ICC/IF)

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DISC1 Antibody (40-6900) in ICC/IF

Immunofluorescence analysis of DISC1 was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with DISC1 Rabbit Polyclonal Antibody (Product # 40-6900) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) ... View More

Product Image Gallery

DISC1 Antibody — Figure 7 DISC1 knockdown and rescue in striatal neurons. ( A ) Example immunoblot showing depletion of endogenous DISC1 detected in PC12 cells using the C-terminal antibody (see Materials and Methods ). The immunoreactive band corresponding to the major full length DISC1 species is shown. ( B ) Example of DISC1 depletion in striatal cultures, detected using the C-terminal (C-term) antibody and verified using the midi antibody, as indicated. ( C ) Quantification of knockdown and rescue phenotypes, based on count of cilia number compiled from 8 independent experiments. ( D ) Examples of the phenotypes observed in the indicated conditions (indicated above each column). The rescue condition (right column of images) shows the localization of DISC1-GFP rather than GFP. Insets indicate relevant regions of the cell body at higher magnification. {KD}

产品信息

40-6900

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:125

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免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫组织化学（悬浮细胞） (IHC (Free))

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免疫细胞化学 (ICC/IF)

1 µg/mL

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from an internal region of the mouse DISC1 (disrupted in schizophrenia 1) isoforms 1 and 2, which differ from rat by one amino acid

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533477

靶标信息

DISC1 (Disrupted in Schizophrenia 1) is a multifunctional protein that interacts with multiple proteins of the centrosome and cytoskeletal system, and is involved in neuronal functions that are dependent upon proper cytoskeletal regulation, including neuronal migration, neurite architecture and intracellular transport. Regions of the brain which express DISC1 include the hippocampus, lateral septum, amygdala, cerebral cortex, cerebellum and paraventricular hypothalamus, and loss of DISC1 function results in differences in structural organization.The DISC1 gene locus is associated with patients afflicted with schizophrenia as a result of chromosomal translocations. DISC-1 encodes a large protein predicted to contain a globular N-terminal domain and a helical C-terminal domain, both of which have the potential to form interactions with other proteins. DISC-1 interacts with proteins involved in the centrosome and cytoskeletal system, including MIPT3, MAP1A and NUDEL; proteins which localize receptors to membranes, including alpha-actinin 2, beta 4-spectrin; and proteins which transduce signals from membrane receptors, including ATF-4 and ATF-5. Therefore, DISC-1 is thought to be involved in intracellular transport, neurite architecture and/or neuronal migration, all of which are thought to be pathogenic in the schizophrenic brain. Decreased expression of DISC1 in neurons caused an accelerated rate of neuronal integration, resulting in aberrant morphological development, suggesting that DISC1 plays a role in dendritic development and synapse formation. DISC-1 is thought to be involved in intracellular transport, neurite architecture and/or neuronal migration, all of which are thought to be pathogenic in the schizophrenic brain.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

An inherited duplication at the gene p21 Protein-Activated Kinase 7 (PAK7) is a risk factor for psychosis.

Human molecular genetics

Morris DW,Pearson RD,Cormican P,Kenny EM,O'Dushlaine CT,Perreault LP,Giannoulatou E,Tropea D,Maher BS,Wormley B,Kelleher E,Fahey C,Molinos I,Bellini S,Pirinen M,Strange A,Freeman C,Thiselton DL,Elves RL,Regan R,Ennis S,Dinan TG,McDonald C,Murphy KC,O'Callaghan E,Waddington JL,Walsh D,O'Donovan M,Grozeva D,Craddock N,Stone J,Scolnick E,Purcell S,Sklar P,Coe B,Eichler EE,Ophoff R,Buizer J,Szatkiewicz J,Hultman C,Sullivan P,Gurling H,Mcquillin A,St Clair D,Rees E,Kirov G,Walters J,Blackwood D,Johnstone M,Donohoe G,O'Neill FA,Kendler KS,Gill M,Riley BP,Spencer CC,Corvin A

40-6900 was used in immunohistochemistry - free floating and western blot to identify rare, highly penetrant risk mutations that contribute to schizophrenia.