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Proteintech

DNA-PKcs Polyclonal Antibody

18 篇参考文献

查看其他40个DNA-PK抗体

说明书

实验方案

技术支持

货号 19983-1-AP

此产品在中国无法购买

150 µL

说明书

实验方案

技术支持

抗体检测数据 (2)

Application

图: 1 / 2

DNA-PKcs Antibody (19983-1-AP) in WB

MCF7 cells were subjected to SDS PAGE followed by western blot with 19983-1-AP (DNA-PKcs antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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产品信息

19983-1-AP

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看14篇已发表文章 14篇已发表文章

免疫组化 (IHC)

Assay-dependent

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Peptide

偶联物

Unconjugated

形式

Liquid

浓度

0.29 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

产品详细信息

The antibody recognizes the C-term of PRKDC.

靶标信息

DNA-PK is a heterotrimeric, DNA-dependent protein kinase that initiates signaling cascades in response to DNA damage. DNA-PK is an atypical kinase, closely related to the PI3 kinases, and is often potently inhibited by small molecules designed to target PI3 kinases. DNA-PK encodes a component of the mediator complex, a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for DNA-PK.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

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CC-115 Mediates GSDME-Dependent Pyroptosis in Lung Adenocarcinoma Through the Akt/Bax Pathway.

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Role of ionizing radiation activated NRF2 in lung cancer radioresistance.

International journal of biological macromolecules

Xu Q,Zhang P,Han X,Ren H,Yu W,Hao W,Luo B,Khan MI,Chen N

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ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer.

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Fri Oct 28 00:00:00 UTC 2022

ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer.

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Zhou C,Li S,Bin K,Qin G,Pan P,Ren D,Zhao Y,Xia W,Chen J,Liu J,Wu H,Zhou Y

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Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.

Nature communications

Wang W,Li J,Tan J,Wang M,Yang J,Zhang ZM,Li C,Basnakian AG,Tang HW,Perrimon N,Zhou Q

Wed Jan 20 00:00:00 UTC 2021

DNA damage promotes microtubule dynamics through a DNA-PK-AKT axis for enhanced repair.

The Journal of cell biology

Ma S,Rong Z,Liu C,Qin X,Zhang X,Chen Q

Mon Feb 01 00:00:00 UTC 2021

DNA damage promotes microtubule dynamics through a DNA-PK-AKT axis for enhanced repair.

The Journal of cell biology

Ma S,Rong Z,Liu C,Qin X,Zhang X,Chen Q

Mon Feb 01 00:00:00 UTC 2021

Targeting Ku86 enhances X-ray-induced radiotherapy sensitivity in serous ovarian cancer cells.

The international journal of biochemistry & cell biology

Ma Q,Kai J,Liu Y,Tong Y,Xie S,Zheng H,Wang Y,Guo L,Lu R

Wed Apr 01 00:00:00 UTC 2020

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Cell death & disease

Guo Z,Wang S,Xie Y,Han Y,Hu S,Guan H,Xie D,Bai C,Liu X,Gu Y,Zhou PK,Ma T

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Rao N,Wang X,Zhai Y,Li J,Xie J,Zhao Y,Ge L

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Ren X,Tang Y,Sun J,Feng J,Chen L,Chen H,Zeng S,Chen C,Li X,Zhu H,Zeng Z

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Fri Jan 24 00:00:00 UTC 2014

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Monroe KM,Yang Z,Johnson JR,Geng X,Doitsh G,Krogan NJ,Greene WC

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生物信息学

蛋白别名: DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNPK1; hyper-radiosensitivity of murine scid mutation, complementing 1; p460

基因别名: DNA-PKcs; DNAPK; DNPK1; HYRC; HYRC1; IMD26; p350; PRKDC; XRCC7

UniProt ID: (Human) P78527

Entrez Gene ID: (Human) 5591

功能

double-stranded DNA binding protein kinase activity protein serine/threonine kinase activity DNA-dependent protein kinase activity protein binding ATP binding transcription factor binding enzyme binding poly(A) RNA binding non-receptor serine/threonine protein kinase protein modifying enzyme

参与通路

somitogenesis negative regulation of protein phosphorylation B cell lineage commitment pro-B cell differentiation T cell lineage commitment negative regulation of immunoglobulin production positive regulation of immune system process double-strand break repair double-strand break repair via nonhomologous end joining cellular protein modification process brain development heart development cell proliferation intrinsic apoptotic signaling pathway in response to DNA damage response to gamma radiation response to activity telomere capping peptidyl-serine phosphorylation protein destabilization positive regulation of type I interferon production cellular response to insulin stimulus T cell differentiation in thymus immunoglobulin V(D)J recombination T cell receptor V(D)J recombination ectopic germ cell programmed cell death regulation of circadian rhythm positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation rhythmic process spleen development thymus development positive regulation of developmental growth regulation of smooth muscle cell proliferation signal transduction involved in mitotic G1 DNA damage checkpoint double-strand break repair via alternative nonhomologous end joining negative regulation of cellular senescence negative regulation of response to gamma radiation